Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F01%3A80000024" target="_blank" >RIV/00027162:_____/01:80000024 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization

Popis výsledku v původním jazyce

The objectives of this study were to develop a two-color fluorescent in situ hybridization (FISH) method for evaluating aneuploidy in gilt oocytes using chromosome-specific DNA probes, and to establish baseline frequencies of aneuploidy in pig oocytes matured in vitro.Only oocytes in which a complementary first polar body was found were confirmed as aneuploid.Thirty-four (3%) of the examined oocytes were aneuploid. Disomy of Chromosome 1 and Chromosome 10 was found in 12/34 and 8/34 of the aneuploid oocytes. Nullisomy of Chromosome 1 and Chromosome 10 was found in 8/34 and 6/34 of the aneuploid oocytes. The frequency of aneuploid oocytes determined by FISH seems to be higher than that determined by conventional methods in other laboratories.

Název v anglickém jazyce

Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization

Popis výsledku anglicky

The objectives of this study were to develop a two-color fluorescent in situ hybridization (FISH) method for evaluating aneuploidy in gilt oocytes using chromosome-specific DNA probes, and to establish baseline frequencies of aneuploidy in pig oocytes matured in vitro.Only oocytes in which a complementary first polar body was found were confirmed as aneuploid.Thirty-four (3%) of the examined oocytes were aneuploid. Disomy of Chromosome 1 and Chromosome 10 was found in 12/34 and 8/34 of the aneuploid oocytes. Nullisomy of Chromosome 1 and Chromosome 10 was found in 8/34 and 6/34 of the aneuploid oocytes. The frequency of aneuploid oocytes determined by FISH seems to be higher than that determined by conventional methods in other laboratories.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F01%2F0291" target="_blank" >GA523/01/0291: Vývoj malovacích DNA sond laserovou mikrodisekcí chromozomů skotu a jejich využití pro diagnostiku chromozomálních aberací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

THERIOGENOLOGY

ISSN

0093-691X

e-ISSN

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Svazek periodika

56

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

771-776

Kód UT WoS článku

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EID výsledku v databázi Scopus

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