Produkce embryí in vitro od býků nositelů robertsonovské translokace 16;20 a 1;29: detekce translokace v embryích pomocí fluorescenční in situ hybridizace

Kód výsledku v IS VaVaI

RIV/00027162:_____/05:06N00402 - isvavai.cz

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robersonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

Popis výsledku v původním jazyce

Robertsonian translocation rob(16;20) in the heterozygous state was discovered in a subfertile bull of the Czech Siemmental breed. The present experiments were designed to detect the rob(16;20) and determine its incidence for in vitro produced embryos(the 6- to 8-cell stage and the blastocyst stage), using fluorescence in situ hybridization (FISH) and the rob(1;29) as a detection control. To characterize semen of both bulls with the rob translocations, their sperm was examined for DNA integrity bythe Sperm Chromatin Structure Assay (SCSA). The embryos were fixed in methanol and acetic acid with Tween 20. Painting probes for chromosomes 16 (sp. Green) and 20 (sp. Orange) and chromosomes 1 (sp. Orange) and 29 (sp. Green) were simultaneouslyhybridized. Inthe embryos derived from the rob(16;20) bull, the presence of this translocation was not detected. On the other hand, 52.5 % of the embryos derived from the rob(1;29) bull were translocation carriers.

Název v anglickém jazyce

Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robersonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

Popis výsledku anglicky

Robertsonian translocation rob(16;20) in the heterozygous state was discovered in a subfertile bull of the Czech Siemmental breed. The present experiments were designed to detect the rob(16;20) and determine its incidence for in vitro produced embryos(the 6- to 8-cell stage and the blastocyst stage), using fluorescence in situ hybridization (FISH) and the rob(1;29) as a detection control. To characterize semen of both bulls with the rob translocations, their sperm was examined for DNA integrity bythe Sperm Chromatin Structure Assay (SCSA). The embryos were fixed in methanol and acetic acid with Tween 20. Painting probes for chromosomes 16 (sp. Green) and 20 (sp. Orange) and chromosomes 1 (sp. Orange) and 29 (sp. Green) were simultaneouslyhybridized. Inthe embryos derived from the rob(16;20) bull, the presence of this translocation was not detected. On the other hand, 52.5 % of the embryos derived from the rob(1;29) bull were translocation carriers.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

QC1197: Využití metod molekulární genetiky pro zefektivnění kontroly dědičnosti hospodářských zvířat

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

31-34

Kód UT WoS článku

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EID výsledku v databázi Scopus

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