Detekce translokace rob(1;29) ve spermiích býků s využitím specifické DNA sondy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000433" target="_blank" >RIV/00027162:_____/08:#0000433 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of translocation rob(1;29) in bull sperm using a specific DNA probe

Popis výsledku v původním jazyce

The Robertsonian translocation rob(1;29), connected with reduced fertility, is widespread in different cattle breeds. After laser microdissection, DOP-PCR, cloning and sequencing, a highly sensitive translocation-specific DNA probe, suitable for detection of rob(1;29) in cattle cells, including spermatozoa was designed. One thousand sperm from each of five heterozygous translocation carrier bulls were analyzed using this probe and a control probe for chromosome 6. Signals of the translocation-specific probe were detected in 48.8%, 50.9%, 50.1%, 51.8%, and 54.8% of spermatozoa, respectively. Semen samples from five control bulls showed only signals of the control probe for chromosome 6. No sperm head with signal of the translocation-specific probe was observed among 1000 spermatozoa from a chimeric (XX/XY) bull, showing 57.5% of 59,XX,rob(1;29) and 42.5% of 60,XY cells in cultured peripheral lymphocytes, demonstrating that female cells do not pass through the process of spermatogenesis.

Název v anglickém jazyce

Detection of translocation rob(1;29) in bull sperm using a specific DNA probe

Popis výsledku anglicky

The Robertsonian translocation rob(1;29), connected with reduced fertility, is widespread in different cattle breeds. After laser microdissection, DOP-PCR, cloning and sequencing, a highly sensitive translocation-specific DNA probe, suitable for detection of rob(1;29) in cattle cells, including spermatozoa was designed. One thousand sperm from each of five heterozygous translocation carrier bulls were analyzed using this probe and a control probe for chromosome 6. Signals of the translocation-specific probe were detected in 48.8%, 50.9%, 50.1%, 51.8%, and 54.8% of spermatozoa, respectively. Semen samples from five control bulls showed only signals of the control probe for chromosome 6. No sperm head with signal of the translocation-specific probe was observed among 1000 spermatozoa from a chimeric (XX/XY) bull, showing 57.5% of 59,XX,rob(1;29) and 42.5% of 60,XY cells in cultured peripheral lymphocytes, demonstrating that female cells do not pass through the process of spermatogenesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytogenetic and genome research

ISSN

1424-8581

e-ISSN

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Svazek periodika

120

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000255779700012

EID výsledku v databázi Scopus

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