Genová exprese u bovinních embryí pocházejících z oocytů s odlišnou vývojovou kompetencí získaných v definovaných stádiích folikulárního vývoje

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F06%3A%230000063" target="_blank" >RIV/00027162:_____/06:#0000063 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/06:00040790

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage

Popis výsledku v původním jazyce

The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different meiotic competence. Oocyte populations were collected and embryos were prepared, either from small or medium follicles, in the growth/stagnation (G/S) or dominance/regression (D/R) stage of the follicular wave. Poly (A)+ mRNA was extracted from blastocysts and the expression of bax-? (Bax) , connexin 43 (Cx 43) and connexin 31 (Cx 31) was estimated using real-time RT-PCR. The relative abundance of Bax and Cx 31 made no significant difference in blastocysts developed from oocytes collected from both medium and small follicles in the G/S or D/R stage. But the relative abundance of the Cx 43 transcript was significantly higher in blastocysts developed from oocytes collected from both medium and small follicles in the G/S stage compared with those in the D/R stage. The relative abundance of Cx 43 can be used as a marker of developmental competence of embryos.

Název v anglickém jazyce

Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage

Popis výsledku anglicky

The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different meiotic competence. Oocyte populations were collected and embryos were prepared, either from small or medium follicles, in the growth/stagnation (G/S) or dominance/regression (D/R) stage of the follicular wave. Poly (A)+ mRNA was extracted from blastocysts and the expression of bax-? (Bax) , connexin 43 (Cx 43) and connexin 31 (Cx 31) was estimated using real-time RT-PCR. The relative abundance of Bax and Cx 31 made no significant difference in blastocysts developed from oocytes collected from both medium and small follicles in the G/S or D/R stage. But the relative abundance of the Cx 43 transcript was significantly higher in blastocysts developed from oocytes collected from both medium and small follicles in the G/S stage compared with those in the D/R stage. The relative abundance of Cx 43 can be used as a marker of developmental competence of embryos.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F02%2F1135" target="_blank" >GA524/02/1135: Vztah exprese genů a vývojové schopnosti časných embryí skotu. Srovnání embryí vyvíjejících se z definovaných oocytů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theriogenology

ISSN

0093-691X

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

7

Kód UT WoS článku

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EID výsledku v databázi Scopus

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