Produkce monoklonálních protilátek proti těžkému řetězci imunoglobulinu kapra obecného (Cyprinus carpio L)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F06%3A%230000081" target="_blank" >RIV/00027162:_____/06:#0000081 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L)

Popis výsledku v původním jazyce

A purification method of carp serum immunoglobulin (IgM) intended for the production of monoclonal antibodies was described in the present study. Hybridomas producing antibodies against IgM heavy chain were selected by the ELISA method and Western blotting. Ascitic fluids were prepared and subsequently tested by the above mentioned methods and typed. Monoclonal antibody with the highest titre of antibodies against carp immunoglobulin was selected for conjugation with horse radish peroxidase. Specificityof the conjugated monoclonal antibody was tested in a complex of various fish species sera. Cross reactivity was not detected in rainbow trout (Oncorhynchus mykiss) and other eleven fish species. Besides common carp, positivity was also found in goldfish (Carassius auratus) and bighead carp (Aristichthys nobilis) that are members of Cyprinidae family. Among fish other than Cyprinidae, positivity was detected in sheatfish (Silurus glanis).

Název v anglickém jazyce

Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L)

Popis výsledku anglicky

A purification method of carp serum immunoglobulin (IgM) intended for the production of monoclonal antibodies was described in the present study. Hybridomas producing antibodies against IgM heavy chain were selected by the ELISA method and Western blotting. Ascitic fluids were prepared and subsequently tested by the above mentioned methods and typed. Monoclonal antibody with the highest titre of antibodies against carp immunoglobulin was selected for conjugation with horse radish peroxidase. Specificityof the conjugated monoclonal antibody was tested in a complex of various fish species sera. Cross reactivity was not detected in rainbow trout (Oncorhynchus mykiss) and other eleven fish species. Besides common carp, positivity was also found in goldfish (Carassius auratus) and bighead carp (Aristichthys nobilis) that are members of Cyprinidae family. Among fish other than Cyprinidae, positivity was detected in sheatfish (Silurus glanis).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/1B44022" target="_blank" >1B44022: Stanovení imunoglobulinů kapra obecného a jejich využití v diagnostice jarní virémie kaprů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

5

Kód UT WoS článku

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EID výsledku v databázi Scopus

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