Korelace laboratorních testů pro detekci imunitní odpovědi na modelu trichofytózy skotu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000184" target="_blank" >RIV/00027162:_____/07:#0000184 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/07:00001557

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A correlation of in vitro tests for the immune response detection: A bovine trichophytosis model

Popis výsledku v původním jazyce

The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease orchallenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. The results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed

Název v anglickém jazyce

A correlation of in vitro tests for the immune response detection: A bovine trichophytosis model

Popis výsledku anglicky

The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease orchallenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. The results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/1B44024" target="_blank" >1B44024: Metody zvýšení účinnosti vakcinačních postupů u hospodářských zvířat</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vaccine

ISSN

0264-410X

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

46

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

7948-7954

Kód UT WoS článku

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EID výsledku v databázi Scopus

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