Cytokinová odpověď prasečích tkáňových kultur na Salmonella enterica serovar Typhimurium a její hilA a ssrA mutanty

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000203" target="_blank" >RIV/00027162:_____/07:#0000203 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokine Response of Porcine Cell Lines to Salmonella enterica serovar Typhimurium and its hilA and ssrA mutants

Popis výsledku v původním jazyce

S. Typhimurium is a facultative intracellular bacterium which can infect pigs. After contact with enterocytes and macrophages, S. Typhimurium induces production of cytokines thus triggering the innate immune response. In this study we evaluated the cytokine response of two porcine cell lines, IPI-2I and 3D4/31, of epithelial or macrophage origins respectively, to the wild type S. Typhimurium and its hilA and ssrA mutants. We observed that the 3D4/31 cell line essentially did not respond to S. Typhimurium infection when a medium with foetal calf serum was used. When the 3D4 cell line was incubated overnight in the presence of porcine serum, it efficiently responded to the wild type strain and the ssrA mutant but not to the noninvasive hilA mutant as measured by mRNA quantification of TNF-alpha, IL-8 and GM-CSF by the real time RT-PCR. In IPI-2I, all the cytokines were induced by the wild type S. Typhimurium and the ssrA mutant while the hilA mutant was unable to induce any of the cytoki

Název v anglickém jazyce

Cytokine Response of Porcine Cell Lines to Salmonella enterica serovar Typhimurium and its hilA and ssrA mutants

Popis výsledku anglicky

S. Typhimurium is a facultative intracellular bacterium which can infect pigs. After contact with enterocytes and macrophages, S. Typhimurium induces production of cytokines thus triggering the innate immune response. In this study we evaluated the cytokine response of two porcine cell lines, IPI-2I and 3D4/31, of epithelial or macrophage origins respectively, to the wild type S. Typhimurium and its hilA and ssrA mutants. We observed that the 3D4/31 cell line essentially did not respond to S. Typhimurium infection when a medium with foetal calf serum was used. When the 3D4 cell line was incubated overnight in the presence of porcine serum, it efficiently responded to the wild type strain and the ssrA mutant but not to the noninvasive hilA mutant as measured by mRNA quantification of TNF-alpha, IL-8 and GM-CSF by the real time RT-PCR. In IPI-2I, all the cytokines were induced by the wild type S. Typhimurium and the ssrA mutant while the hilA mutant was unable to induce any of the cytoki

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1B44020" target="_blank" >1B44020: Patogeneze enterálních koliinfekcí a salmonelóz prasat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zoonoses and Public Health (Journal of Veterinary Medicine B)

ISSN

0931-1793

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

286-293

Kód UT WoS článku

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EID výsledku v databázi Scopus

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