SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000751" target="_blank" >RIV/00027162:_____/11:#0000751 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1297-9716-42-16" target="_blank" >http://dx.doi.org/10.1186/1297-9716-42-16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1297-9716-42-16" target="_blank" >10.1186/1297-9716-42-16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

Popis výsledku v původním jazyce

Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella invasion of host non-professional phagocytes. In addition to invasion, SPI-1-encoded proteins induce cell death of macrophages via activation of caspase-1 which alsocleaves proIL-1 beta and proIL-18, precursors of 2 proinflammatory cytokines. We were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. We infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their SPI-1 deletion mutants. SPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with Delta SPI1 mutants by increased expression of proinflammatory cytokines. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytoki

Název v anglickém jazyce

SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

Popis výsledku anglicky

Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella invasion of host non-professional phagocytes. In addition to invasion, SPI-1-encoded proteins induce cell death of macrophages via activation of caspase-1 which alsocleaves proIL-1 beta and proIL-18, precursors of 2 proinflammatory cytokines. We were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. We infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their SPI-1 deletion mutants. SPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with Delta SPI1 mutants by increased expression of proinflammatory cytokines. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytoki

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Research

ISSN

0928-4249

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

000290659600005

EID výsledku v databázi Scopus

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