Mycobacterium avium subsp. avium studované u přirozeně infikovaného chovu slepic a v prostředí metodami: kultivace, serotypizace a IS901 RFLP

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000204" target="_blank" >RIV/00027162:_____/07:#0000204 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods

Popis výsledku v původním jazyce

Mycobacterium a. avium (MAA; serotype 2, genotype IS901+) was cultured from 21 hens. From a total of 330 samples taken from hens MAA was detected in tissues, in swabs of cloacae and in faeces. Mycobacteria of other species were also isolated. In 7 hens,tuberculous (TB) lesions were found mainly in the liver, spleen and intestine. In 7 hens with TB lesions, Ziehl-Nelsen microscopy revealed acid-fast bacteria (AFB) in only 11 of 94 tissues and in 1 of 7 cloacae swabs. MAA was cultured from 59 of 94 tissues, from 1 of 7 cloacae swabs and from 6 of 7 faeces. In 14 infected hens without TB lesions, AFBs were detected in 3 of 194 tissues. MAA was cultured from 52 of 194 tissues, from 3 of 14 cloacae swabs and from 3 of 14 faeces. DNA of 58 MAA isolates from16 infected hens found to be of three IS901 RFLP types AE, AD and E. From a total of 35 examined environmental samples 4 MAA isolates of RFLP types AE and other 19 isolates of conditionally pathogenic mycobacteria were detected.

Název v anglickém jazyce

Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods

Popis výsledku anglicky

Mycobacterium a. avium (MAA; serotype 2, genotype IS901+) was cultured from 21 hens. From a total of 330 samples taken from hens MAA was detected in tissues, in swabs of cloacae and in faeces. Mycobacteria of other species were also isolated. In 7 hens,tuberculous (TB) lesions were found mainly in the liver, spleen and intestine. In 7 hens with TB lesions, Ziehl-Nelsen microscopy revealed acid-fast bacteria (AFB) in only 11 of 94 tissues and in 1 of 7 cloacae swabs. MAA was cultured from 59 of 94 tissues, from 1 of 7 cloacae swabs and from 6 of 7 faeces. In 14 infected hens without TB lesions, AFBs were detected in 3 of 194 tissues. MAA was cultured from 52 of 194 tissues, from 3 of 14 cloacae swabs and from 3 of 14 faeces. DNA of 58 MAA isolates from16 infected hens found to be of three IS901 RFLP types AE, AD and E. From a total of 35 examined environmental samples 4 MAA isolates of RFLP types AE and other 19 isolates of conditionally pathogenic mycobacteria were detected.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/1B53009" target="_blank" >1B53009: Aviární tuberkulóza a mykobakteriózy: rozvoj metod pro jejich prevenci a tlumení studiem zdrojů infekce a patogeneze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Svazek periodika

127

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

155-164

Kód UT WoS článku

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EID výsledku v databázi Scopus

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