Real-time PCR pro kvantifikaci RNA viru bovinní virové diarhoey pomocí SYBR Green I fluorimetrie

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000227" target="_blank" >RIV/00027162:_____/07:#0000227 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry

Popis výsledku v původním jazyce

Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copiesusing Light Cycler detection system and intercalation fluorogenic dye SYBR Green I. A universal set of primers was selected from a highly conserved 5' untranslated region to detect BVDV type I and II simultaneously. Analysis of 290 bp amplicons enabledmonitoring of the viral RNA/BVDV level in a total of five BVDV strains and sixteen bulk milk samples, and in bovine sera of persistent carriers originating from Czech farms, as well as in a batch of calf serum for cell culture. Melting temperatures of amplicons of BVDV strains of the same genotype group I as the NADL reference strain showed variability of the thermal points, however no significant differences were observed in Tm values between the representatives of genotype group I and

Název v anglickém jazyce

Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry

Popis výsledku anglicky

Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copiesusing Light Cycler detection system and intercalation fluorogenic dye SYBR Green I. A universal set of primers was selected from a highly conserved 5' untranslated region to detect BVDV type I and II simultaneously. Analysis of 290 bp amplicons enabledmonitoring of the viral RNA/BVDV level in a total of five BVDV strains and sixteen bulk milk samples, and in bovine sera of persistent carriers originating from Czech farms, as well as in a batch of calf serum for cell culture. Melting temperatures of amplicons of BVDV strains of the same genotype group I as the NADL reference strain showed variability of the thermal points, however no significant differences were observed in Tm values between the representatives of genotype group I and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/1B44021" target="_blank" >1B44021: Vývoj nových diagnostických metod a jejich aplikace při tlumení produkčních chorob virové etiologie (IBR, BVD-MD).</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

253-261

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—