Quantification of bovine viral diarrhoea virus ribonucleic acid in serum of infected animals by one-step reverse transcriptase quantitative real-time polymerase chain reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000362" target="_blank" >RIV/00027162:_____/19:N0000362 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/19:N0000229

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088040361.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088040361.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201988040361" target="_blank" >10.2754/avb201988040361</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of bovine viral diarrhoea virus ribonucleic acid in serum of infected animals by one-step reverse transcriptase quantitative real-time polymerase chain reaction

Popis výsledku v původním jazyce

Bovine viral diarrhoea virus (BVDV) can cause either acute transient or persistent infection. Identification and removal of persistently infected animals from infected herds is a crucial component to control BVDV infection. Only limited data on serum virus concentration in infected animals are available to date. Using one-step reverse transcriptase quantitative real-time polymerase chain reaction, we quantified the serum viral load in 40 BVDV infected animals. To control nucleic acid extraction, complementary DNA synthesis and polymerase chain reaction amplification, each serum sample was spiked with a known small amount of reference canine coronavirus. Detected ribonucleic acid copy number ranged from 2.2 × 106 to 7.4 × 108 per 1 ml of serum of persistently infected animals and from 6.6 × 104 to 3.3 × 107 of transiently infected animals. These findings support the idea that it is impossible to accurately distinguish between transiently and persistently infected animals just from a single blood sample. To use this testing as a means of declining costs of BVDV control programmes cannot be recommended and paired serum samples have to be investigated to confirm persistent infection.

Název v anglickém jazyce

Quantification of bovine viral diarrhoea virus ribonucleic acid in serum of infected animals by one-step reverse transcriptase quantitative real-time polymerase chain reaction

Popis výsledku anglicky

Bovine viral diarrhoea virus (BVDV) can cause either acute transient or persistent infection. Identification and removal of persistently infected animals from infected herds is a crucial component to control BVDV infection. Only limited data on serum virus concentration in infected animals are available to date. Using one-step reverse transcriptase quantitative real-time polymerase chain reaction, we quantified the serum viral load in 40 BVDV infected animals. To control nucleic acid extraction, complementary DNA synthesis and polymerase chain reaction amplification, each serum sample was spiked with a known small amount of reference canine coronavirus. Detected ribonucleic acid copy number ranged from 2.2 × 106 to 7.4 × 108 per 1 ml of serum of persistently infected animals and from 6.6 × 104 to 3.3 × 107 of transiently infected animals. These findings support the idea that it is impossible to accurately distinguish between transiently and persistently infected animals just from a single blood sample. To use this testing as a means of declining costs of BVDV control programmes cannot be recommended and paired serum samples have to be investigated to confirm persistent infection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

88

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

361-367

Kód UT WoS článku

000509758100001

EID výsledku v databázi Scopus

2-s2.0-85078868618