Monitorování změn integrity chromatinu v populaci motilních bovinních spermií kapacitovaních v podmínkách in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000230" target="_blank" >RIV/00027162:_____/07:#0000230 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/07:00111185

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro

Popis výsledku v původním jazyce

The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring during sperm capacitation stimulated with heparin. Sperm were isolated by Percoll gradient centrifugation from frozen-thawed semen using Tyrode's medium (SP-Talp) and resuspended in a fertilization medium (IVF-Talp). Sperm were incubated at laboratory temperature for 6 h either in IVF-Talp medium with heparin (H+) or without heparin (H-). The results of our study suggest the following outcomes. Separation of motile sperm by Percoll gradient increased the proportion of sperm with undamaged chromatin structure. Sperm incubation induced gentle damage of chromatin integrity which was potentiated by heparin in capacitated sperm. The proportion of sperm with undamaged chromatin structure remained relatively high in the course of capacitation, therefore we can assume it to be high enough for a potential oocyte fertilization.

Název v anglickém jazyce

Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro

Popis výsledku anglicky

The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring during sperm capacitation stimulated with heparin. Sperm were isolated by Percoll gradient centrifugation from frozen-thawed semen using Tyrode's medium (SP-Talp) and resuspended in a fertilization medium (IVF-Talp). Sperm were incubated at laboratory temperature for 6 h either in IVF-Talp medium with heparin (H+) or without heparin (H-). The results of our study suggest the following outcomes. Separation of motile sperm by Percoll gradient increased the proportion of sperm with undamaged chromatin structure. Sperm incubation induced gentle damage of chromatin integrity which was potentiated by heparin in capacitated sperm. The proportion of sperm with undamaged chromatin structure remained relatively high in the course of capacitation, therefore we can assume it to be high enough for a potential oocyte fertilization.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/1B44018" target="_blank" >1B44018: Nové metody predikce fertility býků přispívající k efektivnímu využití reprodukčního potenciálu skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Agriculturae et Silviculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

65-70

Kód UT WoS článku

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EID výsledku v databázi Scopus

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