Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906699" target="_blank" >RIV/62156489:43210/15:43906699 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00027162:_____/15:#0001337
Výsledek na webu
<a href="http://vri.cz/docs/vetmed/60-9-469.pdf" target="_blank" >http://vri.cz/docs/vetmed/60-9-469.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa
Popis výsledku v původním jazyce
This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05
Název v anglickém jazyce
Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa
Popis výsledku anglicky
This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GG - Chov hospodářských zvířat
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LD14104" target="_blank" >LD14104: Vliv stimulace mitochondriální aktivity na vývojový potenciál savčích oocytů</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinární medicína
ISSN
0375-8427
e-ISSN
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Svazek periodika
60
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
7
Strana od-do
469-475
Kód UT WoS článku
000361572400001
EID výsledku v databázi Scopus
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