Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F16%3A10324637" target="_blank" >RIV/00216208:11140/16:10324637 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/rda.12741" target="_blank" >http://dx.doi.org/10.1111/rda.12741</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/rda.12741" target="_blank" >10.1111/rda.12741</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa

Popis výsledku v původním jazyce

Reactive oxygen species (ROS) are fundamental for intracellular signaling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Estrous sheep serum (ESS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored the effects of ESS on ram sperm physiology, especially on ROS production, during in vitro capacitation. Semen samples from 15 rams were cryopreserved. After thawing, samples were submitted to four treatments: Control (CTL), 10% ESS supplementation for in vitro sperm capacitation, caspase inhibitor (INH, Z-VAD-FMK 100 μM), and EES (10%) plus caspase inhibitor (I+E). Sperm samples were incubated for 30 min at 38.5 oC and 5% CO2 and evaluated: motility and kinetic parameters by computer-assisted semen analysis (CASA), and viability (propidium iodide), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), intracellular calcium (FLUO-3), membrane fluidity (M540) and ROS production (CM-H2DCFDA) by flow cytometry. ESS induced changes in kinetic parameters compatible with capacitation, with a decrease in the percentage of progressive motility and linearity, and an increase in the amplitude of the lateral displacement of the sperm head (P<0.05). Moreover, ESS increased the proportion of M540+ viable spermatozoa, YO-PRO-1+ and acrosome reacted spermatozoa (P<0.05). After incubation, ESS and I+E achieved lower ROS levels (P < 0.05). Ca2+ levels did not change with the incubation, but were slightly higher (P<0.05) when both ESS and the inhibitor were present. We suggest that ESS may modulate ROS levels, allowing intracellular signalling for capacitation to occur while preventing higher levels that could trigger apoptosis.

Název v anglickém jazyce

Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa

Popis výsledku anglicky

Reactive oxygen species (ROS) are fundamental for intracellular signaling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Estrous sheep serum (ESS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored the effects of ESS on ram sperm physiology, especially on ROS production, during in vitro capacitation. Semen samples from 15 rams were cryopreserved. After thawing, samples were submitted to four treatments: Control (CTL), 10% ESS supplementation for in vitro sperm capacitation, caspase inhibitor (INH, Z-VAD-FMK 100 μM), and EES (10%) plus caspase inhibitor (I+E). Sperm samples were incubated for 30 min at 38.5 oC and 5% CO2 and evaluated: motility and kinetic parameters by computer-assisted semen analysis (CASA), and viability (propidium iodide), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), intracellular calcium (FLUO-3), membrane fluidity (M540) and ROS production (CM-H2DCFDA) by flow cytometry. ESS induced changes in kinetic parameters compatible with capacitation, with a decrease in the percentage of progressive motility and linearity, and an increase in the amplitude of the lateral displacement of the sperm head (P<0.05). Moreover, ESS increased the proportion of M540+ viable spermatozoa, YO-PRO-1+ and acrosome reacted spermatozoa (P<0.05). After incubation, ESS and I+E achieved lower ROS levels (P < 0.05). Ca2+ levels did not change with the incubation, but were slightly higher (P<0.05) when both ESS and the inhibitor were present. We suggest that ESS may modulate ROS levels, allowing intracellular signalling for capacitation to occur while preventing higher levels that could trigger apoptosis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction in Domestic Animals

ISSN

0936-6768

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

743-750

Kód UT WoS článku

000388334100014

EID výsledku v databázi Scopus

2-s2.0-84986910751