Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F11%3A%230000536" target="_blank" >RIV/26788462:_____/11:#0000536 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49608851:_____/11:#0000495

Výsledek na webu

<a href="http://dx.doi.org/10.4149/gpb_2011_SI1_36" target="_blank" >http://dx.doi.org/10.4149/gpb_2011_SI1_36</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/gpb_2011_SI1_36" target="_blank" >10.4149/gpb_2011_SI1_36</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor

Popis výsledku v původním jazyce

The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100,200 or 400 ng/ml). Following 72-96 h storage at 4 degrees C, the spermatozoa were stained for a plasma membrane integrity (PNA-FITC), membrane phosphatidylserine (PS) translocation (annexin V-Fluos) and apoptosis (Yo-Pro-1), and analyzed by fluorescentmicroscopy. Sperm motility was measured using computer-assisted sperm analysis (CASA) and sperm fertilizing ability was tested using in vitro penetration/fertilization test on bovine prematured oocytes. EGF increased sperm motility at all doses tested,decreased the proportion of spermatozoa with damaged plasma membrane (at 200 or 400 ng/ml), and decreased the proportion of apoptotic (Yo-Pro-1) spermatozoa when given at 200 or 400 (but not 100) ng/ml. The proportion of spermatozoa with

Název v anglickém jazyce

Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor

Popis výsledku anglicky

The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100,200 or 400 ng/ml). Following 72-96 h storage at 4 degrees C, the spermatozoa were stained for a plasma membrane integrity (PNA-FITC), membrane phosphatidylserine (PS) translocation (annexin V-Fluos) and apoptosis (Yo-Pro-1), and analyzed by fluorescentmicroscopy. Sperm motility was measured using computer-assisted sperm analysis (CASA) and sperm fertilizing ability was tested using in vitro penetration/fertilization test on bovine prematured oocytes. EGF increased sperm motility at all doses tested,decreased the proportion of spermatozoa with damaged plasma membrane (at 200 or 400 ng/ml), and decreased the proportion of apoptotic (Yo-Pro-1) spermatozoa when given at 200 or 400 (but not 100) ng/ml. The proportion of spermatozoa with

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QH81324" target="_blank" >QH81324: Rozvoj a využití biotechnologických metod v reprodukci malých přežvýkavců.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENERAL PHYSIOLOGY AND BIOPHYSICS

ISSN

0231-5882

e-ISSN

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Svazek periodika

30

Číslo periodika v rámci svazku

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Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

36-43

Kód UT WoS článku

000293112300005

EID výsledku v databázi Scopus

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