Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F12%3A%230000581" target="_blank" >RIV/49608851:_____/12:#0000581 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S0967199412000500" target="_blank" >http://dx.doi.org/10.1017/S0967199412000500</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0967199412000500" target="_blank" >10.1017/S0967199412000500</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa

Popis výsledku v původním jazyce

The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4?C) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determinedby CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I

Název v anglickém jazyce

Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa

Popis výsledku anglicky

The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4?C) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determinedby CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QH81324" target="_blank" >QH81324: Rozvoj a využití biotechnologických metod v reprodukci malých přežvýkavců.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

říjen 2012

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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