Single Nucleotide Polymorphisms of Interleukin-1 beta Related Genes and their Associations with Infection in the Horse

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000599" target="_blank" >RIV/00027162:_____/08:#0000599 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Single Nucleotide Polymorphisms of Interleukin-1 beta Related Genes and their Associations with Infection in the Horse

Popis výsledku v původním jazyce

In previous work, we found significant associations of horse chromosome 15 microsatellite markers HMSO1 and HTG06 with two horse infections, Rhodococcus equi and Lawsonia intracellularis, respectively. Interleukin-1 beta subunit and interleukin-1 receptor antagonist encoding genes (IL1B and IL1RN) could be considered as candidate genes. Therefore, we identified single nucleotide polymorphisms (SNPs) within three interleukin-1 beta functionally related genes: IL1B, IL1RN and Casp1 (interleukin-1 beta converting enzyme/caspasel encoding gene). Using appropriate restriction fragment length polymorphism (PCR-RFLP) and/or single strand conformation polymorphism (PCR-SSCP) markers, their associations with the two infections by genotyping foals from the original study were tested. A statistically significant association between an intronic SNP of the Casp1 gene with R. equi infection was found. The IL1RN gene was physically localized by fluorescence in-situ hybridization to 15q13-q14.

Název v anglickém jazyce

Single Nucleotide Polymorphisms of Interleukin-1 beta Related Genes and their Associations with Infection in the Horse

Popis výsledku anglicky

In previous work, we found significant associations of horse chromosome 15 microsatellite markers HMSO1 and HTG06 with two horse infections, Rhodococcus equi and Lawsonia intracellularis, respectively. Interleukin-1 beta subunit and interleukin-1 receptor antagonist encoding genes (IL1B and IL1RN) could be considered as candidate genes. Therefore, we identified single nucleotide polymorphisms (SNPs) within three interleukin-1 beta functionally related genes: IL1B, IL1RN and Casp1 (interleukin-1 beta converting enzyme/caspasel encoding gene). Using appropriate restriction fragment length polymorphism (PCR-RFLP) and/or single strand conformation polymorphism (PCR-SSCP) markers, their associations with the two infections by genotyping foals from the original study were tested. A statistically significant association between an intronic SNP of the Casp1 gene with R. equi infection was found. The IL1RN gene was physically localized by fluorescence in-situ hybridization to 15q13-q14.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F06%2F1402" target="_blank" >GA523/06/1402: Geny imunitní odpovědi čeledi Equidae: genetická diversita a komparativní genomika</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Animal Genomics for Animal Health

ISBN

978-3-8055-8619-1

Počet stran výsledku

5

Strana od-do

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Počet stran knihy

440

Název nakladatele

Karger

Místo vydání

Basel, Switzerland

Kód UT WoS kapitoly

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