aroA and aroD mutations influence biofilm formation in Salmonella Enteritidis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000502" target="_blank" >RIV/00027162:_____/09:#0000502 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

aroA and aroD mutations influence biofilm formation in Salmonella Enteritidis

Popis výsledku v původním jazyce

aro mutants of Salmonella enterica are frequently used as live vaccines for oral vaccination of domestic animals. Interestingly, besides their auxotrophy, they appear to be of reduced resistance to the components of the innate immune response due to a defect in outer membrane and/or cell wall integrity. We were interested in the ability of aroA and aroD mutants of S. Enteritidis to adhere to solid surfaces. aroA and aroD mutants did not adhere to solid surfaces although they bind Congo red and producedD-mannose and D-glucose capsular polysaccharides in the same amount as the wild type strain. However the aro mutants exhibited a decreased production of cellulose, N-acetyl-D-glucosamine or N-acetylneuraminic acid containing capsular polysaccharide and fimbriae which explains their inability to form biofilms. aroA and aroD containing plasmids complemented all the defects of the aro mutants. Beside its attenuation for host, the loss of ability to form biofilm is additional characteristic

Název v anglickém jazyce

aroA and aroD mutations influence biofilm formation in Salmonella Enteritidis

Popis výsledku anglicky

aro mutants of Salmonella enterica are frequently used as live vaccines for oral vaccination of domestic animals. Interestingly, besides their auxotrophy, they appear to be of reduced resistance to the components of the innate immune response due to a defect in outer membrane and/or cell wall integrity. We were interested in the ability of aroA and aroD mutants of S. Enteritidis to adhere to solid surfaces. aroA and aroD mutants did not adhere to solid surfaces although they bind Congo red and producedD-mannose and D-glucose capsular polysaccharides in the same amount as the wild type strain. However the aro mutants exhibited a decreased production of cellulose, N-acetyl-D-glucosamine or N-acetylneuraminic acid containing capsular polysaccharide and fimbriae which explains their inability to form biofilms. aroA and aroD containing plasmids complemented all the defects of the aro mutants. Beside its attenuation for host, the loss of ability to form biofilm is additional characteristic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06030" target="_blank" >LC06030: Biomolekulární centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Svazek periodika

291

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000262224700005

EID výsledku v databázi Scopus

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