Aro mutace u Salmonella enterica způsobují defekt v buněčné stěně a integritě vnější membrány

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000352" target="_blank" >RIV/00027162:_____/08:#0000352 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/08:00050960

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity

Popis výsledku v původním jazyce

In this study we characterized aro mutants of Salmonella Enteritidis and Typhimurium which are frequently used as live oral vaccines. We found that the aroA, aroD and aroC mutants were sensitive to blood serum, albumen, EDTA and ovotransferrin, and thisdefect could be complemented by appropriate aro gene cloned in plasmid. Subsequent microarray analysis of gene expression in aroD mutant in S. Typhimurium indicated that reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid and such overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

Název v anglickém jazyce

Aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity

Popis výsledku anglicky

In this study we characterized aro mutants of Salmonella Enteritidis and Typhimurium which are frequently used as live oral vaccines. We found that the aroA, aroD and aroC mutants were sensitive to blood serum, albumen, EDTA and ovotransferrin, and thisdefect could be complemented by appropriate aro gene cloned in plasmid. Subsequent microarray analysis of gene expression in aroD mutant in S. Typhimurium indicated that reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid and such overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06030" target="_blank" >LC06030: Biomolekulární centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal Bacteriology

ISSN

0021-9193

e-ISSN

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Svazek periodika

190

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000255202100008

EID výsledku v databázi Scopus

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