Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000531" target="_blank" >RIV/00027162:_____/09:#0000531 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential

Popis výsledku v původním jazyce

If any new live Salmonella vaccine is introduced in the future, it is quite probable that detailed characterisation of attenuation will be required. In this study we compared 34 isogenic mutants of S. Enteritidis in aroA, aroD, galE, ssrA, sseA, phoP, rpoS, ompR, htrA, clpP, lon, rfaL, rfaG, rfaC, hfq, sodCI, hilA, sipA, avrA, sopB, sopA, sopE, sifA, shdA, fliC, fur, relA, spoT, relA-spoT, misL, rmbA, STM4258, STM4259 and spvBC genes for their resistance to stresses and for their virulence and immunogenicity in Balb/C mice. We found that the cold and bile resistances did not correlate with the resistances to other stresses. Resistance to acid pH, heat, polymyxin and serum correlated with each other and also with the attenuation. When the residual virulence and immunogenicity were both considered, mutants in htrA, ompR, aroA, aroD and lon performed the best in mice. Furthermore, when a comparison of polymyxin and serum sensitive mutants was performed, the serum sensitive mutants were mo

Název v anglickém jazyce

Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential

Popis výsledku anglicky

If any new live Salmonella vaccine is introduced in the future, it is quite probable that detailed characterisation of attenuation will be required. In this study we compared 34 isogenic mutants of S. Enteritidis in aroA, aroD, galE, ssrA, sseA, phoP, rpoS, ompR, htrA, clpP, lon, rfaL, rfaG, rfaC, hfq, sodCI, hilA, sipA, avrA, sopB, sopA, sopE, sifA, shdA, fliC, fur, relA, spoT, relA-spoT, misL, rmbA, STM4258, STM4259 and spvBC genes for their resistance to stresses and for their virulence and immunogenicity in Balb/C mice. We found that the cold and bile resistances did not correlate with the resistances to other stresses. Resistance to acid pH, heat, polymyxin and serum correlated with each other and also with the attenuation. When the residual virulence and immunogenicity were both considered, mutants in htrA, ompR, aroA, aroD and lon performed the best in mice. Furthermore, when a comparison of polymyxin and serum sensitive mutants was performed, the serum sensitive mutants were mo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/QH91238" target="_blank" >QH91238: Vakcinace a ochrana drůbeže před salmonelami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vaccine

ISSN

0264-410X

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

38

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000269071900018

EID výsledku v databázi Scopus

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