The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000551" target="_blank" >RIV/00027162:_____/09:#0000551 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization

Popis výsledku v původním jazyce

This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcinemacrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2.

Název v anglickém jazyce

The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization

Popis výsledku anglicky

This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcinemacrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Research

ISSN

0928-4249

e-ISSN

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Svazek periodika

40

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000272039500013

EID výsledku v databázi Scopus

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