2,2 ',4,4 ',5,5 '-Hexachlorobiphenyl (PCB 153) induces degradation of adherens junction proteins and inhibits beta-catenin-dependent transcription in liver epithelial cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000569" target="_blank" >RIV/00027162:_____/09:#0000569 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/09:00327032

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

2,2 ',4,4 ',5,5 '-Hexachlorobiphenyl (PCB 153) induces degradation of adherens junction proteins and inhibits beta-catenin-dependent transcription in liver epithelial cells

Popis výsledku v původním jazyce

In this study we analyzed the effects of 2,2´,4,4´,5,5´-hexachlorobiphenyl (PCB 153) on proteins involved in the formation of adherens junctions in WB-F344 cells. PCB 153 induced a gradual degradation of E-adherin, beta-catenin or plakoglobin proteins. PCB 153 had no effect on mRNA levels of the above mentioned proteins. PCB 153 also decreased levels of the active beta-catenin form, which is the key co-activator of Wnt-induced TCF/LEF-dependent gene expression and reduced Axin2 mRNA, a known Wnt signaling transcriptional target. Nevertheless, PCB 153 had no effect on phosphorylation of glycogen synthase kinase-3beta, which is supposed to target beta-catenin for its proteasomal degradation. Taken together, the present data suggest that PCB 153 may interfere with functions of adherens junction proteins involved in both cell-to-cell communication and intracellular signaling. Such mechanisms might be involved in the effects of non-dioxin-like PCBs contributing to liver tumor promotion.

Název v anglickém jazyce

2,2 ',4,4 ',5,5 '-Hexachlorobiphenyl (PCB 153) induces degradation of adherens junction proteins and inhibits beta-catenin-dependent transcription in liver epithelial cells

Popis výsledku anglicky

In this study we analyzed the effects of 2,2´,4,4´,5,5´-hexachlorobiphenyl (PCB 153) on proteins involved in the formation of adherens junctions in WB-F344 cells. PCB 153 induced a gradual degradation of E-adherin, beta-catenin or plakoglobin proteins. PCB 153 had no effect on mRNA levels of the above mentioned proteins. PCB 153 also decreased levels of the active beta-catenin form, which is the key co-activator of Wnt-induced TCF/LEF-dependent gene expression and reduced Axin2 mRNA, a known Wnt signaling transcriptional target. Nevertheless, PCB 153 had no effect on phosphorylation of glycogen synthase kinase-3beta, which is supposed to target beta-catenin for its proteasomal degradation. Taken together, the present data suggest that PCB 153 may interfere with functions of adherens junction proteins involved in both cell-to-cell communication and intracellular signaling. Such mechanisms might be involved in the effects of non-dioxin-like PCBs contributing to liver tumor promotion.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F06%2F0517" target="_blank" >GA524/06/0517: Mechanismy disrupce mezibuněčné komunikace a regulace buněčné proliferace v jaterních buňkách</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology

ISSN

0300-483X

e-ISSN

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Svazek periodika

260

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000267161000013

EID výsledku v databázi Scopus

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