Ectopic Activation of Wnt/beta-Catenin Signaling in Lens Fiber Cells Results in Cataract Formation and Aberrant Fiber Cell Differentiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10159326" target="_blank" >RIV/00216208:11310/13:10159326 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078279" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078279</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0078279" target="_blank" >10.1371/journal.pone.0078279</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ectopic Activation of Wnt/beta-Catenin Signaling in Lens Fiber Cells Results in Cataract Formation and Aberrant Fiber Cell Differentiation

Popis výsledku v původním jazyce

The Wnt/beta-catenin signaling pathway controls many processes during development, including cell proliferation, cell differentiation and tissue homeostasis, and its aberrant regulation has been linked to various pathologies. In this study we investigated the effect of ectopic activation of Wnt/beta-catenin signaling during lens fiber cell differentiation. To activate Wnt/beta-catenin signaling in lens fiber cells, the transgenic mouse referred to as alpha A-CLEF was generated, in which the transactivation domain of beta-catenin was fused to the DNA-binding protein LEF1, and expression of the transgene was controlled by alpha A-crystallin promoter. Constitutive activation of Wnt/beta-catenin signaling in lens fiber cells of alpha A-CLEF mice resulted in abnormal and delayed fiber cell differentiation. Moreover, adult alpha A-CLEF mice developed cataract, microphthalmia and manifested downregulated levels of gamma-crystallins in lenses. We provide evidence of aberrant expression of cell

Název v anglickém jazyce

Ectopic Activation of Wnt/beta-Catenin Signaling in Lens Fiber Cells Results in Cataract Formation and Aberrant Fiber Cell Differentiation

Popis výsledku anglicky

The Wnt/beta-catenin signaling pathway controls many processes during development, including cell proliferation, cell differentiation and tissue homeostasis, and its aberrant regulation has been linked to various pathologies. In this study we investigated the effect of ectopic activation of Wnt/beta-catenin signaling during lens fiber cell differentiation. To activate Wnt/beta-catenin signaling in lens fiber cells, the transgenic mouse referred to as alpha A-CLEF was generated, in which the transactivation domain of beta-catenin was fused to the DNA-binding protein LEF1, and expression of the transgene was controlled by alpha A-crystallin promoter. Constitutive activation of Wnt/beta-catenin signaling in lens fiber cells of alpha A-CLEF mice resulted in abnormal and delayed fiber cell differentiation. Moreover, adult alpha A-CLEF mice developed cataract, microphthalmia and manifested downregulated levels of gamma-crystallins in lenses. We provide evidence of aberrant expression of cell

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FF - ORL, oftalmologie, stomatologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

000326334500108

EID výsledku v databázi Scopus

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