Ectopic activation of Wnt/beta-catenin sgnaling in lens fiber cells results in cataract formation and aberrant fiber cell differentiation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422571" target="_blank" >RIV/68378050:_____/13:00422571 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1371/journal.pone.0078279" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0078279</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0078279" target="_blank" >10.1371/journal.pone.0078279</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Ectopic activation of Wnt/beta-catenin sgnaling in lens fiber cells results in cataract formation and aberrant fiber cell differentiation
Popis výsledku v původním jazyce
The Wnt/beta-catenin signaling pathway controls many processes during development, including cell proliferation, cell differentiation and tissue homeostasis, and its aberrant regulation has been linked to various pathologies. In this study we investigated the effect of ectopic activation of Wnt/beta-catenin signaling during lens fiber cell differentiation. To activate Wnt/beta-catenin signaling in lens fiber cells, the transgenic mouse referred to as alpha A-CLEF was generated, in which the transactivation domain of beta-catenin was fused to the DNA-binding protein LEF1, and expression of the transgene was controlled by alpha A-crystallin promoter. Constitutive activation of Wnt/beta-catenin signaling in lens fiber cells of alpha A-CLEF mice resulted in abnormal and delayed fiber cell differentiation. Moreover, adult alpha A-CLEF mice developed cataract, microphthalmia and manifested downregulated levels of gamma-crystallins in lenses. We provide evidence of aberrant expression of cell
Název v anglickém jazyce
Ectopic activation of Wnt/beta-catenin sgnaling in lens fiber cells results in cataract formation and aberrant fiber cell differentiation
Popis výsledku anglicky
The Wnt/beta-catenin signaling pathway controls many processes during development, including cell proliferation, cell differentiation and tissue homeostasis, and its aberrant regulation has been linked to various pathologies. In this study we investigated the effect of ectopic activation of Wnt/beta-catenin signaling during lens fiber cell differentiation. To activate Wnt/beta-catenin signaling in lens fiber cells, the transgenic mouse referred to as alpha A-CLEF was generated, in which the transactivation domain of beta-catenin was fused to the DNA-binding protein LEF1, and expression of the transgene was controlled by alpha A-crystallin promoter. Constitutive activation of Wnt/beta-catenin signaling in lens fiber cells of alpha A-CLEF mice resulted in abnormal and delayed fiber cell differentiation. Moreover, adult alpha A-CLEF mice developed cataract, microphthalmia and manifested downregulated levels of gamma-crystallins in lenses. We provide evidence of aberrant expression of cell
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS ONE
ISSN
1932-6203
e-ISSN
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Svazek periodika
8
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
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Kód UT WoS článku
000326334500108
EID výsledku v databázi Scopus
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