Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000761" target="_blank" >RIV/00027162:_____/11:#0000761 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle

Popis výsledku v původním jazyce

Products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected cattle were examined for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Viable MAP cells were detected using culture in fermentors for up to 2 months; the presence of MAP DNA was demonstrated in all anaerobic fermentors and digestate up to 16 months after initiation of work at a biogas plant, using IS900 real time PCR (qPCR). According to these results, a fermentation process that extended beyond 2 months removed all viable MAP cells and therefore rendered its product MAP free. MAP DNA was found during all the examined periods, which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined.

Název v anglickém jazyce

Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle

Popis výsledku anglicky

Products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected cattle were examined for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Viable MAP cells were detected using culture in fermentors for up to 2 months; the presence of MAP DNA was demonstrated in all anaerobic fermentors and digestate up to 16 months after initiation of work at a biogas plant, using IS900 real time PCR (qPCR). According to these results, a fermentation process that extended beyond 2 months removed all viable MAP cells and therefore rendered its product MAP free. MAP DNA was found during all the examined periods, which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ISSN

0099-2240

e-ISSN

—

Svazek periodika

77

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

3115-3119

Kód UT WoS článku

000289773100036

EID výsledku v databázi Scopus

—