Production and analytical characterization of neopterin immunoreagents for biosensor developments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000766" target="_blank" >RIV/00027162:_____/11:#0000766 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production and analytical characterization of neopterin immunoreagents for biosensor developments

Popis výsledku v původním jazyce

Neopterin is a valuable biomarker of cellular immunity associated with various pathological situations Novel immunogens were prepared to produce functional immunoreagents.The prepared monoclonal antibody reached in both direct and indirect ELISA format LoD of 0.18 and 0.45 mu g L-1, respectively, and was a superior immunoreagent for immunoassay developments. The best polyclonal antibody was more sensitive in direct ELISA with LoD of 0.05 mu g L-1. A satisfactory correlation of results was found betweenthe polyclonal ELISA and IBL Neopterin ELISA kit within the plasma concentration range 0.5-16 mu g L-1 (R = 0.874; n = 40), and slightly lower correlation was found for monoclonal-based ELISA (R = 0.819; n = 40). These data show that the antibodies may be used as analytical reagents for the integration into multianalyte biochip detection systems.

Název v anglickém jazyce

Production and analytical characterization of neopterin immunoreagents for biosensor developments

Popis výsledku anglicky

Neopterin is a valuable biomarker of cellular immunity associated with various pathological situations Novel immunogens were prepared to produce functional immunoreagents.The prepared monoclonal antibody reached in both direct and indirect ELISA format LoD of 0.18 and 0.45 mu g L-1, respectively, and was a superior immunoreagent for immunoassay developments. The best polyclonal antibody was more sensitive in direct ELISA with LoD of 0.05 mu g L-1. A satisfactory correlation of results was found betweenthe polyclonal ELISA and IBL Neopterin ELISA kit within the plasma concentration range 0.5-16 mu g L-1 (R = 0.874; n = 40), and slightly lower correlation was found for monoclonal-based ELISA (R = 0.819; n = 40). These data show that the antibodies may be used as analytical reagents for the integration into multianalyte biochip detection systems.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

399

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GE - Gruzie

Počet stran výsledku

8

Strana od-do

979-986

Kód UT WoS článku

000285781900044

EID výsledku v databázi Scopus

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