Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000847" target="_blank" >RIV/00027162:_____/11:#0000847 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test

Popis výsledku v původním jazyce

Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, thedegree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant eventhough the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.

Název v anglickém jazyce

Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test

Popis výsledku anglicky

Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, thedegree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant eventhough the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů