Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000882" target="_blank" >RIV/00027162:_____/12:#0000882 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.tvjl.2012.05.002" target="_blank" >http://dx.doi.org/10.1016/j.tvjl.2012.05.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tvjl.2012.05.002" target="_blank" >10.1016/j.tvjl.2012.05.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture

Popis výsledku v původním jazyce

Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterialnumbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the d

Název v anglickém jazyce

Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture

Popis výsledku anglicky

Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterialnumbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the d

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Journal

ISSN

1090-0233

e-ISSN

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Svazek periodika

194

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

354-360

Kód UT WoS článku

000313224300021

EID výsledku v databázi Scopus

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