Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001128" target="_blank" >RIV/00027162:_____/14:#0001128 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.tvjl.2014.05.032" target="_blank" >http://dx.doi.org/10.1016/j.tvjl.2014.05.032</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tvjl.2014.05.032" target="_blank" >10.1016/j.tvjl.2014.05.032</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR
Popis výsledku v původním jazyce
Mycobacterium avium subsp. paratuberculosis (MAP) has a high degree of resistance to chemical and physical procedures frequently used for the elimination of other bacteria. Recently, a method for the determination of viability by exposure of MAP to propidium monoazide (PMA) and subsequent real time quantitative PCR (qPCR) was established and found to be comparable with culture. The aim of this study was to apply the PMA qPCR method to determine the impact of increasing concentration or time and repeatedcycles of the application of selected disinfectants on MAP viability. Different MAP isolates responded to the same type of stress in different ways. The laboratory strain CAPM 6381 had the highest tolerance, while the 8819 low-passage field isolate wasthe most sensitive. Ultraviolet exposure caused only a partial reduction in MAP viability; all MAP isolates were relatively resistant to chlorine. Only the application of peracetic acid led to the total elimination of MAP. Repeated applic
Název v anglickém jazyce
Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR
Popis výsledku anglicky
Mycobacterium avium subsp. paratuberculosis (MAP) has a high degree of resistance to chemical and physical procedures frequently used for the elimination of other bacteria. Recently, a method for the determination of viability by exposure of MAP to propidium monoazide (PMA) and subsequent real time quantitative PCR (qPCR) was established and found to be comparable with culture. The aim of this study was to apply the PMA qPCR method to determine the impact of increasing concentration or time and repeatedcycles of the application of selected disinfectants on MAP viability. Different MAP isolates responded to the same type of stress in different ways. The laboratory strain CAPM 6381 had the highest tolerance, while the 8819 low-passage field isolate wasthe most sensitive. Ultraviolet exposure caused only a partial reduction in MAP viability; all MAP isolates were relatively resistant to chlorine. Only the application of peracetic acid led to the total elimination of MAP. Repeated applic
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GJ - Choroby a škůdci zvířat, veterinární medicina
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinary journal
ISSN
1090-0233
e-ISSN
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Svazek periodika
201
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
6
Strana od-do
359-364
Kód UT WoS článku
000342711600022
EID výsledku v databázi Scopus
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