Prevalence of antibiotic resistance genes in faecal samples from cattle, pigs and poultry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230000976" target="_blank" >RIV/00027162:_____/13:#0000976 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence of antibiotic resistance genes in faecal samples from cattle, pigs and poultry

Popis výsledku v původním jazyce

Antibiotic resistant bacteria can be easily isolated from the faeces of cattle, pigs or poultry. However, whether the production of different farm animals is associated with a higher or lower prevalence of antibiotic resistance is not clear. In this study we therefore used real time PCR for the quantification of antibiotic gene prevalence in the DNA purified from the faeces of farm animals. First we showed that experimental streptomycin therapy of 12-week-old chickens and 46-week-old hens significantlyincreased the relative prevalence of strA and sul2 genes though this did not necessarily indicate an absolute increase of strA-encoding bacteria. Next we quantified antibiotic gene prevalence in the DNA purified from the faeces of cattle, pigs and layinghens. The lowest prevalence of strA, aadA, sul1, sul2, tet(A), tet(B), tet(G) and cat genes was recorded in the intestinal contents of laying hens. In cattle and pig faecal samples, an intermediate prevalence of antibiotic resistance gen

Název v anglickém jazyce

Prevalence of antibiotic resistance genes in faecal samples from cattle, pigs and poultry

Popis výsledku anglicky

Antibiotic resistant bacteria can be easily isolated from the faeces of cattle, pigs or poultry. However, whether the production of different farm animals is associated with a higher or lower prevalence of antibiotic resistance is not clear. In this study we therefore used real time PCR for the quantification of antibiotic gene prevalence in the DNA purified from the faeces of farm animals. First we showed that experimental streptomycin therapy of 12-week-old chickens and 46-week-old hens significantlyincreased the relative prevalence of strA and sul2 genes though this did not necessarily indicate an absolute increase of strA-encoding bacteria. Next we quantified antibiotic gene prevalence in the DNA purified from the faeces of cattle, pigs and layinghens. The lowest prevalence of strA, aadA, sul1, sul2, tet(A), tet(B), tet(G) and cat genes was recorded in the intestinal contents of laying hens. In cattle and pig faecal samples, an intermediate prevalence of antibiotic resistance gen

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VETERINARNI MEDICINA

ISSN

0375-8427

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

298-304

Kód UT WoS článku

000322515300002

EID výsledku v databázi Scopus

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