Virus quantitation by transmission electron microscopy, TCID50, and the role of timing virus harvesting: A case study of three animal viruses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230000988" target="_blank" >RIV/00027162:_____/13:#0000988 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.jviromet.2013.04.008" target="_blank" >http://dx.doi.org/10.1016/j.jviromet.2013.04.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jviromet.2013.04.008" target="_blank" >10.1016/j.jviromet.2013.04.008</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Virus quantitation by transmission electron microscopy, TCID50, and the role of timing virus harvesting: A case study of three animal viruses
Popis výsledku v původním jazyce
Quantitation of viruses is practised widely in both basic and applied virology. Infectious titration in cell cultures, the most common approach to it, is quite labour-intensive and alternative protocols are therefore sought. One of the alternatives is transmission electron microscope (TEM) quantitation using latex particles at a known concentration as a reference for counting virus particles. If virus TCID50 is determined in parallel, the ratio of infectious to non-infectious virus particles may be established. This study employs such an approach to compute the number of virus particles and TCID50, and establish their correlation for three viruses: Canine adenovirus 1 (CAdV-1), Feline calicivirus (FCV) and Bovine herpesvirus 1 (BoHV-1). Each of the viruses was grown in five replicates until complete cytopathology was recorded (time 0), then frozen. They were thawed, filter-sterilised and left for additional periods of 16,32 and 48 hat 37 degrees C. At each time point, TCID50 was charac
Název v anglickém jazyce
Virus quantitation by transmission electron microscopy, TCID50, and the role of timing virus harvesting: A case study of three animal viruses
Popis výsledku anglicky
Quantitation of viruses is practised widely in both basic and applied virology. Infectious titration in cell cultures, the most common approach to it, is quite labour-intensive and alternative protocols are therefore sought. One of the alternatives is transmission electron microscope (TEM) quantitation using latex particles at a known concentration as a reference for counting virus particles. If virus TCID50 is determined in parallel, the ratio of infectious to non-infectious virus particles may be established. This study employs such an approach to compute the number of virus particles and TCID50, and establish their correlation for three viruses: Canine adenovirus 1 (CAdV-1), Feline calicivirus (FCV) and Bovine herpesvirus 1 (BoHV-1). Each of the viruses was grown in five replicates until complete cytopathology was recorded (time 0), then frozen. They were thawed, filter-sterilised and left for additional periods of 16,32 and 48 hat 37 degrees C. At each time point, TCID50 was charac
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Virological Methods
ISSN
0166-0934
e-ISSN
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Svazek periodika
191
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
5
Strana od-do
136-140
Kód UT WoS článku
000321154800008
EID výsledku v databázi Scopus
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