Determination of African swine fever virus viability in meat during long-term storage and sous-vide cooking using cell culture and real-time PCR combined with palladium compound pre-treatment methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000010" target="_blank" >RIV/00027162:_____/23:N0000010 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/92/1/0053/" target="_blank" >https://actavet.vfu.cz/92/1/0053/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202392010053" target="_blank" >10.2754/avb202392010053</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of African swine fever virus viability in meat during long-term storage and sous-vide cooking using cell culture and real-time PCR combined with palladium compound pre-treatment methods

Popis výsledku v původním jazyce

African swine fever virus is causative agent of an acute and highly contagious disease affecting domestic and wild members of the family Suidae. The virus can be transmitted by direct contact among infected animals or via a contaminated environment or feed. Since the contaminated meat or products thereof have been characterised as the most probable vehicle in several outbreaks, the aim of present study was to define viability of the virus in meat under conditions of freezing and chilling (-25 °C and 6 °C) and low temperature cooking (55 °C for 2.5 hours and for 1 hour). Two independent methods were employed; cell culture as a reference and real-time polymerase chain reaction combined with palladium compounds (BB-PdCl2 and PdCl2COD) pre-treatment as an alternative method. Obtained results demonstrated minimal decrease in infectious virus titter during the storage at -25 °C and remaining dose of viruses in meat stored at 6 °C for 14 months that can cause a disease after ingestion. The result obtained by both methods applied on samples corresponded to each other. In contrast, results related to the virus persistence during thermal-treated meat indicated that its stability is much lower than previously thought; the infectious viruses were not detected after the treatment at 55 °C for 1 hour by infectivity assay. The observed difference of one order of magnitude of virus detected using palladium compounds pre-treatment suggests presence of intact rather than infectious viruses. The better suitability of PdCl2COD than BB-PdCl2 pre-treatment was demonstrated.

Název v anglickém jazyce

Determination of African swine fever virus viability in meat during long-term storage and sous-vide cooking using cell culture and real-time PCR combined with palladium compound pre-treatment methods

Popis výsledku anglicky

African swine fever virus is causative agent of an acute and highly contagious disease affecting domestic and wild members of the family Suidae. The virus can be transmitted by direct contact among infected animals or via a contaminated environment or feed. Since the contaminated meat or products thereof have been characterised as the most probable vehicle in several outbreaks, the aim of present study was to define viability of the virus in meat under conditions of freezing and chilling (-25 °C and 6 °C) and low temperature cooking (55 °C for 2.5 hours and for 1 hour). Two independent methods were employed; cell culture as a reference and real-time polymerase chain reaction combined with palladium compounds (BB-PdCl2 and PdCl2COD) pre-treatment as an alternative method. Obtained results demonstrated minimal decrease in infectious virus titter during the storage at -25 °C and remaining dose of viruses in meat stored at 6 °C for 14 months that can cause a disease after ingestion. The result obtained by both methods applied on samples corresponded to each other. In contrast, results related to the virus persistence during thermal-treated meat indicated that its stability is much lower than previously thought; the infectious viruses were not detected after the treatment at 55 °C for 1 hour by infectivity assay. The observed difference of one order of magnitude of virus detected using palladium compounds pre-treatment suggests presence of intact rather than infectious viruses. The better suitability of PdCl2COD than BB-PdCl2 pre-treatment was demonstrated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/QK1920113" target="_blank" >QK1920113: Virus afrického moru prasat v mase a masných výrobcích – metody detekce a studium perzistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

92

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

53-59

Kód UT WoS článku

000933978400008

EID výsledku v databázi Scopus

2-s2.0-85147815938