A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000076" target="_blank" >RIV/00027162:_____/23:N0000076 - isvavai.cz</a>
Výsledek na webu
<a href="https://journals.pan.pl/dlibra/publication/145024/edition/127674/content" target="_blank" >https://journals.pan.pl/dlibra/publication/145024/edition/127674/content</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.24425/pjvs.2023.145024" target="_blank" >10.24425/pjvs.2023.145024</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
Popis výsledku v původním jazyce
Cultivation-based assays represent the gold standard for the assessment of the virus infectivity; however, they are time-consuming and not suitable for each virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. Present study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, with the attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl2-COD) produced the highest differences found between native and heat-treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 minutes, 4 °C) were applied on the ASFV and the heat inactivation profile of ASFV was assessed. Significant decrease of detected quantity of ASFV DNA after heat-treatment (60 °C and 95 °C) and consequent incubation with Pd compounds was observed. BB-PdCl2 and PdCl2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses like BoHV-1 or ASFV.
Název v anglickém jazyce
A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
Popis výsledku anglicky
Cultivation-based assays represent the gold standard for the assessment of the virus infectivity; however, they are time-consuming and not suitable for each virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. Present study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, with the attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl2-COD) produced the highest differences found between native and heat-treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 minutes, 4 °C) were applied on the ASFV and the heat inactivation profile of ASFV was assessed. Significant decrease of detected quantity of ASFV DNA after heat-treatment (60 °C and 95 °C) and consequent incubation with Pd compounds was observed. BB-PdCl2 and PdCl2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses like BoHV-1 or ASFV.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10607 - Virology
Návaznosti výsledku
Projekt
<a href="/cs/project/QK1920113" target="_blank" >QK1920113: Virus afrického moru prasat v mase a masných výrobcích – metody detekce a studium perzistence</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Polish Journal of Veterinary Science
ISSN
1505-1773
e-ISSN
2300-2557
Svazek periodika
26
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
11
Strana od-do
211-221
Kód UT WoS článku
001023483300005
EID výsledku v databázi Scopus
2-s2.0-85164230067