The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001151" target="_blank" >RIV/00027162:_____/14:#0001151 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jam.12654" target="_blank" >http://dx.doi.org/10.1111/jam.12654</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.12654" target="_blank" >10.1111/jam.12654</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage

Popis výsledku v původním jazyce

Methods and ResultsTwo virus strains from each of six animal virus families, including both enveloped and nonenveloped viruses, were lyophilized in (i) culture medium, (ii) with the addition of gelatine-sucrose and (iii) skim milk-sodium glutamate. All the virus suspensions were frozen (i) at -80 degrees C or (ii) in liquid nitrogen before lyophilization. Virus titre assay after lyophilization and after 8months storage at 4 degrees C revealed that the efficacy of stabilizers depended on virus structure.Generally, the best protective quality for enveloped viruses was achieved with gelatine-sucrose, which best maintained their infectivity and envelope morphology. Even additive-free culture medium proved adequate for nonenveloped viruses. Differences instabilizer efficacy were also found between virus families and were expressed immediately after lyophilization; the activity of stabilizers in the course of storage was very similar. Freezing in liquid nitrogen proved beneficial for picor

Název v anglickém jazyce

The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage

Popis výsledku anglicky

Methods and ResultsTwo virus strains from each of six animal virus families, including both enveloped and nonenveloped viruses, were lyophilized in (i) culture medium, (ii) with the addition of gelatine-sucrose and (iii) skim milk-sodium glutamate. All the virus suspensions were frozen (i) at -80 degrees C or (ii) in liquid nitrogen before lyophilization. Virus titre assay after lyophilization and after 8months storage at 4 degrees C revealed that the efficacy of stabilizers depended on virus structure.Generally, the best protective quality for enveloped viruses was achieved with gelatine-sucrose, which best maintained their infectivity and envelope morphology. Even additive-free culture medium proved adequate for nonenveloped viruses. Differences instabilizer efficacy were also found between virus families and were expressed immediately after lyophilization; the activity of stabilizers in the course of storage was very similar. Freezing in liquid nitrogen proved beneficial for picor

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

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Svazek periodika

117

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1810-1819

Kód UT WoS článku

000345510300026

EID výsledku v databázi Scopus

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