In Vitro and in Silico Derived Relative Effect Potencies of Ah-Receptor-Mediated Effects by PCDD/Fs and PCBs in Rat, Mouse, and Guinea Pig CALUX Cell Lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001175" target="_blank" >RIV/00027162:_____/14:#0001175 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1021/tx5001255" target="_blank" >http://dx.doi.org/10.1021/tx5001255</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/tx5001255" target="_blank" >10.1021/tx5001255</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In Vitro and in Silico Derived Relative Effect Potencies of Ah-Receptor-Mediated Effects by PCDD/Fs and PCBs in Rat, Mouse, and Guinea Pig CALUX Cell Lines
Popis výsledku v původním jazyce
Species-specific relative effect potencies (REPs), responses of dioxin-like compounds (DLCs), were assessed using chemical-activated luciferase gene expression assays derived from guinea pig, rat, and mouse cell lines. Almost all 20 congeners tested in the rodent cell lines were partial agonists and less efficacious than 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). REPs were calculated for each congener using concentrations at which 20% of the maximal TCDD response was reached; the REP values obtained for PCDD/Fs were comparable with their toxic equivalency factors assigned by the World Health Organization (WHO-TEF), while those for PCBs were in general lower than the WHO-TEF values. The guinea pig cell line was the most sensitive as indicated by the 20% effect concentrations of TCDD of 1.5, 5.6, and 11.0 pM for guinea pig, rat, and mouse cells, respectively. In order to provide REP values for all WHO-TEF assigned compounds, quantitative structure activity relationship (QSAR) models wer
Název v anglickém jazyce
In Vitro and in Silico Derived Relative Effect Potencies of Ah-Receptor-Mediated Effects by PCDD/Fs and PCBs in Rat, Mouse, and Guinea Pig CALUX Cell Lines
Popis výsledku anglicky
Species-specific relative effect potencies (REPs), responses of dioxin-like compounds (DLCs), were assessed using chemical-activated luciferase gene expression assays derived from guinea pig, rat, and mouse cell lines. Almost all 20 congeners tested in the rodent cell lines were partial agonists and less efficacious than 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). REPs were calculated for each congener using concentrations at which 20% of the maximal TCDD response was reached; the REP values obtained for PCDD/Fs were comparable with their toxic equivalency factors assigned by the World Health Organization (WHO-TEF), while those for PCBs were in general lower than the WHO-TEF values. The guinea pig cell line was the most sensitive as indicated by the 20% effect concentrations of TCDD of 1.5, 5.6, and 11.0 pM for guinea pig, rat, and mouse cells, respectively. In order to provide REP values for all WHO-TEF assigned compounds, quantitative structure activity relationship (QSAR) models wer
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FR - Farmakologie a lékárnická chemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chemical Research in Toxicology
ISSN
0893-228X
e-ISSN
—
Svazek periodika
27
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
1120-1132
Kód UT WoS článku
000339462700005
EID výsledku v databázi Scopus
—