Evaluation of DNA extraction methods for PCR-based detection of Listeria monocytogenes from vegetables

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001256" target="_blank" >RIV/00027162:_____/15:#0001256 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X" target="_blank" >http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/lam.12367" target="_blank" >10.1111/lam.12367</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of DNA extraction methods for PCR-based detection of Listeria monocytogenes from vegetables

Popis výsledku v původním jazyce

Epidemiological data indicate that raw vegetables are associated with outbreaks of Listeria monocytogenes. Therefore, there is a demand for the availability of rapid and sensitive methods, such as PCR assays, for the detection and accurate discriminationof L. monocytogenes. However, the efficiency of PCR methods can be negatively affected by inhibitory compounds commonly found in vegetable matrices that may cause false-negative results. Therefore, the sample processing and DNA isolation steps must be carefully evaluated prior to the introduction of such methods into routine practice. In this study, we compared the ability of three column-based and four magnetic bead-based commercial DNA isolation kits to extract DNA of the model micro-organism L. monocytogenes from raw vegetables. The DNA isolation efficiency of all isolation kits was determined using a triplex real-time qPCR assay designed to specifically detect L. monocytogenes. The kit with best performance, the PowerSoil Microbial

Název v anglickém jazyce

Evaluation of DNA extraction methods for PCR-based detection of Listeria monocytogenes from vegetables

Popis výsledku anglicky

Epidemiological data indicate that raw vegetables are associated with outbreaks of Listeria monocytogenes. Therefore, there is a demand for the availability of rapid and sensitive methods, such as PCR assays, for the detection and accurate discriminationof L. monocytogenes. However, the efficiency of PCR methods can be negatively affected by inhibitory compounds commonly found in vegetable matrices that may cause false-negative results. Therefore, the sample processing and DNA isolation steps must be carefully evaluated prior to the introduction of such methods into routine practice. In this study, we compared the ability of three column-based and four magnetic bead-based commercial DNA isolation kits to extract DNA of the model micro-organism L. monocytogenes from raw vegetables. The DNA isolation efficiency of all isolation kits was determined using a triplex real-time qPCR assay designed to specifically detect L. monocytogenes. The kit with best performance, the PowerSoil Microbial

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Letters in Applied Microbiology

ISSN

0266-8254

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

265-272

Kód UT WoS článku

000335981900015

EID výsledku v databázi Scopus

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