Tick salivary sialostatin L2 suppresses IFN responses in mouse dendritic cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3AN0000007" target="_blank" >RIV/00027162:_____/15:N0000007 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/pim.12162" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/pim.12162</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pim.12162" target="_blank" >10.1111/pim.12162</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tick salivary sialostatin L2 suppresses IFN responses in mouse dendritic cells

Popis výsledku v původním jazyce

Type I interferon (IFN), mainly produced by dendritic cells(DCs), is critical in the host defence against tick-transmittedpathogens. Here, we report that salivary cysteine proteaseinhibitor from the hard tick Ixodes scapularis, sialostatin L2,affects IFN-bmediated immune reactions in mouse dendriticcells. Following IFN receptor ligation, the Janus activatedkinases/signal transducer and activator of transcription(JAK/STAT) pathway is activated. We show that sialostatinL2 attenuates phosphorylation of STATs in spleen dendriticcells upon addition of recombinant IFN-b. LPS-stimulateddendritic cells release IFN-bwhich in turn leads to the induc-tion of IFN-stimulated genes (ISG) through JAK/STATpathway activation. The induction of two ISG, interferon reg-ulatory factor 7 (IRF-7) and IP-10, was suppressed by sia-lostatin L2 in LPS-stimulated dendritic cells. Finally, theinterference of sialostatin L2 with IFN action led to theenhanced replication of tick-borne encephalitis virus in DC.In summary, we present here that tick salivary cystatin nega-tively affects IFN-bresponses which may consequentlyincrease the pathogen load after transmission via tick saliva.

Název v anglickém jazyce

Tick salivary sialostatin L2 suppresses IFN responses in mouse dendritic cells

Popis výsledku anglicky

Type I interferon (IFN), mainly produced by dendritic cells(DCs), is critical in the host defence against tick-transmittedpathogens. Here, we report that salivary cysteine proteaseinhibitor from the hard tick Ixodes scapularis, sialostatin L2,affects IFN-bmediated immune reactions in mouse dendriticcells. Following IFN receptor ligation, the Janus activatedkinases/signal transducer and activator of transcription(JAK/STAT) pathway is activated. We show that sialostatinL2 attenuates phosphorylation of STATs in spleen dendriticcells upon addition of recombinant IFN-b. LPS-stimulateddendritic cells release IFN-bwhich in turn leads to the induc-tion of IFN-stimulated genes (ISG) through JAK/STATpathway activation. The induction of two ISG, interferon reg-ulatory factor 7 (IRF-7) and IP-10, was suppressed by sia-lostatin L2 in LPS-stimulated dendritic cells. Finally, theinterference of sialostatin L2 with IFN action led to theenhanced replication of tick-borne encephalitis virus in DC.In summary, we present here that tick salivary cystatin nega-tively affects IFN-bresponses which may consequentlyincrease the pathogen load after transmission via tick saliva.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasite immunology

ISSN

0141-9838

e-ISSN

1365-3024

Svazek periodika

37

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

70-78

Kód UT WoS článku

000348718100002

EID výsledku v databázi Scopus

2-s2.0-84921404085