Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected on farms and from supermarkets in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000021" target="_blank" >RIV/00027162:_____/16:N0000021 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0168160515301501" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0168160515301501</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2015.10.017" target="_blank" >10.1016/j.ijfoodmicro.2015.10.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected on farms and from supermarkets in the Czech Republic

Popis výsledku v původním jazyce

The Cronobacter genus (previously known as Enterobacter sakazakii) comprises seven species (C. sakazakii, C. malonaticus, C. muytjensii, C. turicensis, C. dublinensis, C. universalis and C. condimenti) which cause serious infections in neonates and immunocompromised people. Most of the documented outbreaks of these bacteria have been associated with consumption of contaminated powdered infant formula. The plant environment is considered to be the natural habitat of these bacteria. Therefore, a total number of 563 samples of vegetables, fruit, water and environmental swabs were collected from local farms and supermarkets in the Czech Republic and investigated for the presence of Cronobacter spp. The obtained 45 isolates (8.0%) were further characterized by phenotyping (antimicrobial resistance, capsule and pigment production) and genotyping (fusA sequencing, MLST, PCR-serotyping) methods. Most of the Cronobacter isolates (42.2%) were identified as C. sakazakii, followed by C. turicensis (31.1%), C. dublinensis (22.2%), C. malonaticus (2.2%) and C. universalis (2.2%). The 25 identified sequence types, out of which 17 were unique for only one strain, indicated a high diversity of strains. C. sakazakii sequence type 4 (ST 4), which has been associated with many cases of meningitis, was isolated only in one case. A strong association of C. turicensis and C. dublinensis with the plant environment can be deduced from our results.

Název v anglickém jazyce

Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected on farms and from supermarkets in the Czech Republic

Popis výsledku anglicky

The Cronobacter genus (previously known as Enterobacter sakazakii) comprises seven species (C. sakazakii, C. malonaticus, C. muytjensii, C. turicensis, C. dublinensis, C. universalis and C. condimenti) which cause serious infections in neonates and immunocompromised people. Most of the documented outbreaks of these bacteria have been associated with consumption of contaminated powdered infant formula. The plant environment is considered to be the natural habitat of these bacteria. Therefore, a total number of 563 samples of vegetables, fruit, water and environmental swabs were collected from local farms and supermarkets in the Czech Republic and investigated for the presence of Cronobacter spp. The obtained 45 isolates (8.0%) were further characterized by phenotyping (antimicrobial resistance, capsule and pigment production) and genotyping (fusA sequencing, MLST, PCR-serotyping) methods. Most of the Cronobacter isolates (42.2%) were identified as C. sakazakii, followed by C. turicensis (31.1%), C. dublinensis (22.2%), C. malonaticus (2.2%) and C. universalis (2.2%). The 25 identified sequence types, out of which 17 were unique for only one strain, indicated a high diversity of strains. C. sakazakii sequence type 4 (ST 4), which has been associated with many cases of meningitis, was isolated only in one case. A strong association of C. turicensis and C. dublinensis with the plant environment can be deduced from our results.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Food Microbiology

ISSN

0168-1605

e-ISSN

—

Svazek periodika

217

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

130-136

Kód UT WoS článku

000366767600018

EID výsledku v databázi Scopus

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