Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000036" target="_blank" >RIV/00027162:_____/16:N0000036 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/16:00089528

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s00604-015-1697-z" target="_blank" >http://link.springer.com/article/10.1007/s00604-015-1697-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-015-1697-z" target="_blank" >10.1007/s00604-015-1697-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays

Popis výsledku v původním jazyce

We report that nanocrystalline Prussian blue of the type Fe-4[Fe(CN)(6)](3) is a powerful peroxidase mimic for use in labeling of biomolecules. The cubic nanocrystals typically have a diameter of 15 nm and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form an intensively colored product with an absorption maximum at 662 nm. The determined pseudo turnover number is similar to 20,000 s(-1) which is the highest value reported for nanoparticles of a size comparable to common proteins. We also present a method for the biotinylation of the surface of these nanocrystals, and show their use in competitive bioaffinity based assays of biotin and human serum albumin. The limits of detection are 0.35 and 0.27 mu g mL(-1), respectively. The results prove the applicability of coordination polymers for signal amplification and also their compatibility with the format of enzyme linked immunosorbent assays.

Název v anglickém jazyce

Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays

Popis výsledku anglicky

We report that nanocrystalline Prussian blue of the type Fe-4[Fe(CN)(6)](3) is a powerful peroxidase mimic for use in labeling of biomolecules. The cubic nanocrystals typically have a diameter of 15 nm and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form an intensively colored product with an absorption maximum at 662 nm. The determined pseudo turnover number is similar to 20,000 s(-1) which is the highest value reported for nanoparticles of a size comparable to common proteins. We also present a method for the biotinylation of the surface of these nanocrystals, and show their use in competitive bioaffinity based assays of biotin and human serum albumin. The limits of detection are 0.35 and 0.27 mu g mL(-1), respectively. The results prove the applicability of coordination polymers for signal amplification and also their compatibility with the format of enzyme linked immunosorbent assays.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchimica Acta

ISSN

0026-3672

e-ISSN

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Svazek periodika

183

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

8

Strana od-do

651-658

Kód UT WoS článku

000370176500017

EID výsledku v databázi Scopus

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