Dynamics of mono- and dual-species biofilm formation and interactions between Staphylococcus aureus and Gram-negative bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000112" target="_blank" >RIV/00027162:_____/17:N0000112 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12705/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12705/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1751-7915.12705" target="_blank" >10.1111/1751-7915.12705</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of mono- and dual-species biofilm formation and interactions between Staphylococcus aureus and Gram-negative bacteria

Popis výsledku v původním jazyce

Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72h was studied. Biofilm formation was evaluated by the crystal violet assay (CV), enumeration of colony-forming units (CFUcm(-2)) and visualization using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In general, Gram-negative bacterial species and S.aureus interacted in a competitive manner. The tested Gram-negative bacteria grew better in mixed dual-species biofilms than in their mono-species biofilms as determined using the CV assay, CFUml(-2) enumeration, and CLSM and SEM visualization. In contrast, the growth of S.aureus biofilms was reduced when cultured in dual-species biofilms. CLSM images revealed grape-like clusters of S.aureus and monolayers of Gram-negative bacteria in both mono- and dual-species biofilms. S.aureus clusters in dual-species biofilms were significantly smaller than clusters in S.aureus mono-species biofilms.

Název v anglickém jazyce

Dynamics of mono- and dual-species biofilm formation and interactions between Staphylococcus aureus and Gram-negative bacteria

Popis výsledku anglicky

Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72h was studied. Biofilm formation was evaluated by the crystal violet assay (CV), enumeration of colony-forming units (CFUcm(-2)) and visualization using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In general, Gram-negative bacterial species and S.aureus interacted in a competitive manner. The tested Gram-negative bacteria grew better in mixed dual-species biofilms than in their mono-species biofilms as determined using the CV assay, CFUml(-2) enumeration, and CLSM and SEM visualization. In contrast, the growth of S.aureus biofilms was reduced when cultured in dual-species biofilms. CLSM images revealed grape-like clusters of S.aureus and monolayers of Gram-negative bacteria in both mono- and dual-species biofilms. S.aureus clusters in dual-species biofilms were significantly smaller than clusters in S.aureus mono-species biofilms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Biotechnology

ISSN

1751-7915

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

819-832

Kód UT WoS článku

000404123100016

EID výsledku v databázi Scopus

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