Použití konfokální a dvoufotonové excitační mikroskopie pro studium Testate Amoebae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F08%3A12148202" target="_blank" >RIV/68407700:21460/08:12148202 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae

Popis výsledku v původním jazyce

Testate amoebae (Protozoa:Rhizopoda) are a group of unicellular animals (20 - 400 ?m), protected with the shell (SiO2, CaCO3, proteins), living in fresh water habitats. They are used as model organisms in population ecology, ecotoxicology, paleoecology -thanks to their cosmopolitan disperse and species-specific ecological preferences together with low ecological valence to the changes of the environmental surroundings. Testate amoebae are usually examined using scanning electron microscopy (SEM) and environmental SEM (E-SEM). Scanning electron microscope allows for much better resolution than confocal laser scanning microscopy (CLSM) far sufficient for recognizing determinant features), but is limited to viewing surface of the specimen. For this reason, we use CLSM and two-photon excitation (TPE) fluorescence microscopy to visualize the shell and inner structures of testate amoebae. We tried as many as 15 fluorescent dyes.

Název v anglickém jazyce

The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae

Popis výsledku anglicky

Testate amoebae (Protozoa:Rhizopoda) are a group of unicellular animals (20 - 400 ?m), protected with the shell (SiO2, CaCO3, proteins), living in fresh water habitats. They are used as model organisms in population ecology, ecotoxicology, paleoecology -thanks to their cosmopolitan disperse and species-specific ecological preferences together with low ecological valence to the changes of the environmental surroundings. Testate amoebae are usually examined using scanning electron microscopy (SEM) and environmental SEM (E-SEM). Scanning electron microscope allows for much better resolution than confocal laser scanning microscopy (CLSM) far sufficient for recognizing determinant features), but is limited to viewing surface of the specimen. For this reason, we use CLSM and two-photon excitation (TPE) fluorescence microscopy to visualize the shell and inner structures of testate amoebae. We tried as many as 15 fluorescent dyes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

53

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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