Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications forTaxonomy and Ecophysiology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F10%3A00172888" target="_blank" >RIV/68407700:21460/10:00172888 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/10:00354405 RIV/67985823:_____/10:00354405

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications forTaxonomy and Ecophysiology

Popis výsledku v původním jazyce

Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. TA taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or scanning electron microscopy (SEM/ESEM).We explored the potential applications of confocal laser scanning microscopy (CLSM), two photon excitation microscopy (TPEM), phase contrast, differential interference contrast (DIC Nomarski), and polarization microscopy to visualize TA shells and innerstructures of living cells, which is not possible by SEM or environmental SEM. Images captured by CLSM and TPEM were utilized to create three-dimensional (3D) visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems. This approach broadens the understanding of TA cell and shell morphology, and inner structures including organelles and endosymbionts, with potential implications in taxonomy and ecophysiology.

Název v anglickém jazyce

Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications forTaxonomy and Ecophysiology

Popis výsledku anglicky

Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. TA taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or scanning electron microscopy (SEM/ESEM).We explored the potential applications of confocal laser scanning microscopy (CLSM), two photon excitation microscopy (TPEM), phase contrast, differential interference contrast (DIC Nomarski), and polarization microscopy to visualize TA shells and innerstructures of living cells, which is not possible by SEM or environmental SEM. Images captured by CLSM and TPEM were utilized to create three-dimensional (3D) visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems. This approach broadens the understanding of TA cell and shell morphology, and inner structures including organelles and endosymbionts, with potential implications in taxonomy and ecophysiology.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000284715300010

EID výsledku v databázi Scopus

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