Foodborne bacterial, viral and protozoan pathogens in field and market strawberries and environment of strawberry farms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000015" target="_blank" >RIV/00027162:_____/18:N0000015 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/18:00104942

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/1750-3841.14401" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/1750-3841.14401</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1750-3841.14401" target="_blank" >10.1111/1750-3841.14401</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Foodborne bacterial, viral and protozoan pathogens in field and market strawberries and environment of strawberry farms

Popis výsledku v původním jazyce

Field-grown strawberries, the environment of strawberry farms and fresh strawberries from marketplaces were examined for bacterial, viral and protozoan pathogens. The presence of bacteria was determined using culture and real time PCR (qPCR), presence of protozoa and viruses using qPCR and reverse transcription qPCR, respectively. The highest proportion of positivity was observed for E. coli both in field and purchased strawberries (up to 48.6%). Finding of Cronobacter ranged from 0.6 to 9% both for field and market strawberries. The prevalence of other pathogens (L. monocytogenes, G. intestinalis, Cryptosporidium sp. and Norovirus) in strawberries was below 4.5%; HAV was not detected at all. Positivity of the environment was determined to be lower than 2.1% for all microorganisms, except for E. coli. The concentration of pathogens in most samples did not exceed 100 CFU/g using culture and 1.8 × 102 GE/g of strawberries or 6.1 × 102 GE/ml of environmental samples using qPCR. All studied farms applied preventive measures such as drip irrigation, avoidance of organic fertilisers, and use of mulch foils or gloves for workers to decrease contamination of strawberries. Despite this, pathogens were found in fresh strawberries. Even at low concentrations, these pathogens can be a source of infection for consumers. Thus, their presence in strawberries is of particular significance as these are mostly consumed fresh and without any thermal processing.

Název v anglickém jazyce

Foodborne bacterial, viral and protozoan pathogens in field and market strawberries and environment of strawberry farms

Popis výsledku anglicky

Field-grown strawberries, the environment of strawberry farms and fresh strawberries from marketplaces were examined for bacterial, viral and protozoan pathogens. The presence of bacteria was determined using culture and real time PCR (qPCR), presence of protozoa and viruses using qPCR and reverse transcription qPCR, respectively. The highest proportion of positivity was observed for E. coli both in field and purchased strawberries (up to 48.6%). Finding of Cronobacter ranged from 0.6 to 9% both for field and market strawberries. The prevalence of other pathogens (L. monocytogenes, G. intestinalis, Cryptosporidium sp. and Norovirus) in strawberries was below 4.5%; HAV was not detected at all. Positivity of the environment was determined to be lower than 2.1% for all microorganisms, except for E. coli. The concentration of pathogens in most samples did not exceed 100 CFU/g using culture and 1.8 × 102 GE/g of strawberries or 6.1 × 102 GE/ml of environmental samples using qPCR. All studied farms applied preventive measures such as drip irrigation, avoidance of organic fertilisers, and use of mulch foils or gloves for workers to decrease contamination of strawberries. Despite this, pathogens were found in fresh strawberries. Even at low concentrations, these pathogens can be a source of infection for consumers. Thus, their presence in strawberries is of particular significance as these are mostly consumed fresh and without any thermal processing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Science

ISSN

0022-1147

e-ISSN

1750-3841

Svazek periodika

83

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

3069-3075

Kód UT WoS článku

000452797900022

EID výsledku v databázi Scopus

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