Progress in sublingual immunisation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000034" target="_blank" >RIV/00027162:_____/18:N0000034 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Progress in sublingual immunisation

Popis výsledku v původním jazyce

11th International Conference Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants and Modern Drug Delivery Systems, Telč, 5.-7.6.2018 – lecture. Introduction: Effectiveness, safety and low cost of application, short time needed for development, low cost of production are factors driven development of modern vaccines. We combined advanced nanomaterials like nanofibre-based textile, nanoliposomes, technology of their production (electrospinning, microfluidic mixing, microprinting) and advanced organic synthetic chemistry to develop an system for construction of non-invasive vaccines, especially for sublingual application. Material and Methods: Electrospinning together with spraying were used for preparation of nano-fibre-based mucoadhesive films. Self-assembling nanoliposomal platform for construction of vaccination nanoparticles were produced by microfluidic mixing or lipid film hydration. Various types of antigen (e.g. proteoliposomes, VLP, viruses, pDNA, bacterial ghosts) were tested to be incorporated into nanofibre mesh. Printing of nanoliposome-based vaccination particles onto nanofibre-based layer has been used. TEM, SEM, confocal microscopy, AFM, DLS were used to characterise various formulations. Pig model was used to study penetration of nanoparticles into submucosal tissues and lymph nodes. Immune response was studied in mice. Results: Nanofibre-based mucoadhesive films were found to be compatible with various antigen formulation. No irritation was observed in pigs, mice and human. Adhered nanoparticles were released quantitatively and were able to penetrate into sublingual tissue and reached draining lymph nodes. Systemic and mucosal immune response was induced against HIV-1 derived recombinant antigen in mice immunised sublingually. Proteoliposomes labelled with GFP were printed onto nanofibre-based films to demonstrate feasibility of the technology. Discussion: Nanofibre-based mucoadhesive films were developed for safe sublingual vaccination and application of nanopartice-based drugs. Technology of “Printed vaccines” was designed and tested. The whole technology has a potential for development and industrial production of vaccines for mucosal/transdermal vaccination via non-invasive routes. Data from mice experiments are very supportive in favour of this technology. Preclinical testing of various antigens (influenza, HIV-1, Zika virus, papilloma virus) is in progress on pig model.

Název v anglickém jazyce

Progress in sublingual immunisation

Popis výsledku anglicky

11th International Conference Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants and Modern Drug Delivery Systems, Telč, 5.-7.6.2018 – lecture. Introduction: Effectiveness, safety and low cost of application, short time needed for development, low cost of production are factors driven development of modern vaccines. We combined advanced nanomaterials like nanofibre-based textile, nanoliposomes, technology of their production (electrospinning, microfluidic mixing, microprinting) and advanced organic synthetic chemistry to develop an system for construction of non-invasive vaccines, especially for sublingual application. Material and Methods: Electrospinning together with spraying were used for preparation of nano-fibre-based mucoadhesive films. Self-assembling nanoliposomal platform for construction of vaccination nanoparticles were produced by microfluidic mixing or lipid film hydration. Various types of antigen (e.g. proteoliposomes, VLP, viruses, pDNA, bacterial ghosts) were tested to be incorporated into nanofibre mesh. Printing of nanoliposome-based vaccination particles onto nanofibre-based layer has been used. TEM, SEM, confocal microscopy, AFM, DLS were used to characterise various formulations. Pig model was used to study penetration of nanoparticles into submucosal tissues and lymph nodes. Immune response was studied in mice. Results: Nanofibre-based mucoadhesive films were found to be compatible with various antigen formulation. No irritation was observed in pigs, mice and human. Adhered nanoparticles were released quantitatively and were able to penetrate into sublingual tissue and reached draining lymph nodes. Systemic and mucosal immune response was induced against HIV-1 derived recombinant antigen in mice immunised sublingually. Proteoliposomes labelled with GFP were printed onto nanofibre-based films to demonstrate feasibility of the technology. Discussion: Nanofibre-based mucoadhesive films were developed for safe sublingual vaccination and application of nanopartice-based drugs. Technology of “Printed vaccines” was designed and tested. The whole technology has a potential for development and industrial production of vaccines for mucosal/transdermal vaccination via non-invasive routes. Data from mice experiments are very supportive in favour of this technology. Preclinical testing of various antigens (influenza, HIV-1, Zika virus, papilloma virus) is in progress on pig model.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Farmakologie, Imunoterapie, nanoToxikologie)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů