Intradermal vaccination combined with oil-based adjuvants: Dendritic cell activation and priming of T-cell response

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000052" target="_blank" >RIV/00027162:_____/18:N0000052 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intradermal vaccination combined with oil-based adjuvants: Dendritic cell activation and priming of T-cell response

Popis výsledku v původním jazyce

To determine the effect of i.d. immunization with oil-based adjuvants on dendritic cell activation and T-cell polarization, two in vivo experiments were performed. Firstly, animals were immunized with model antigen KLH only or KLH combined with different adjuvants: complete or incomplete Freund's adjuvants, Montanide ISA, Emulsigen and Al(OH)3. Blood samples were taken before immunization, three weeks after immunization and two weeks after reimmunization. Levels of IgG1 and IgG2 antibodies in sera were determined by ELISA. Oil-based adjuvants induced high IgG2 primary response suggesting high Th1 cell polarization. The second study was performed to determine the role of dendritic cells on T-cell polarization after i.d. vaccination, local cytokine and chemokine response was tested. Animals were immunized with the same antigen-adjuvant combination and skin biopsies were taken 4 and 24 hours post-immunization for histopathological analyses and quantitative RT-PCR. As expected, oil - based adjuvants induced rapid Th1- priming dendritic cell activation.

Název v anglickém jazyce

Intradermal vaccination combined with oil-based adjuvants: Dendritic cell activation and priming of T-cell response

Popis výsledku anglicky

To determine the effect of i.d. immunization with oil-based adjuvants on dendritic cell activation and T-cell polarization, two in vivo experiments were performed. Firstly, animals were immunized with model antigen KLH only or KLH combined with different adjuvants: complete or incomplete Freund's adjuvants, Montanide ISA, Emulsigen and Al(OH)3. Blood samples were taken before immunization, three weeks after immunization and two weeks after reimmunization. Levels of IgG1 and IgG2 antibodies in sera were determined by ELISA. Oil-based adjuvants induced high IgG2 primary response suggesting high Th1 cell polarization. The second study was performed to determine the role of dendritic cells on T-cell polarization after i.d. vaccination, local cytokine and chemokine response was tested. Animals were immunized with the same antigen-adjuvant combination and skin biopsies were taken 4 and 24 hours post-immunization for histopathological analyses and quantitative RT-PCR. As expected, oil - based adjuvants induced rapid Th1- priming dendritic cell activation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů