Future of multiplexing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000068" target="_blank" >RIV/00027162:_____/18:N0000068 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/18:00102708

Výsledek na webu

<a href="https://hd2018.natur.cuni.cz/programme" target="_blank" >https://hd2018.natur.cuni.cz/programme</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Future of multiplexing

Popis výsledku v původním jazyce

24th Helminthological Days. xMAP technology based on magnetic microspheres represents an open architecture platform, capable of development and adaptation into many multiplex detection assays. Technology is beneficial to end users and therefore it is utilized in various pharmaceutical, clinical and research laboratories not only for nucleic acid assays but also for protein assay formats. On the market, there are available few commercial detection panels, however, these are mainly meant for major clinically important pathogens, infectious diseases or human health. Our research has been focused to fill in the market gaps and develop an in-house assay suitable for detection of genomic DNA of food-borne pathogens (e.g. Trichinella spiralis, Toxoplasma gondii, Giardia intestinalis, Yersinia enterocolitica, Campylobacter jejuni, Salmonella enterica) in various food-related matrices and design the diagnostic system based on the recombinant proteins characteristic for pathogen infections (e.g. Trichinella spp.). Up to this date, we have optimized the working protocol for highly specific multiplex oligonucleotide ligation PCR (MOL-PCR) together with MagPix analysis intended for multiplex detection panel for food-borne pathogens, which is currently still being expanded. The protein assay is under optimization.

Název v anglickém jazyce

Future of multiplexing

Popis výsledku anglicky

24th Helminthological Days. xMAP technology based on magnetic microspheres represents an open architecture platform, capable of development and adaptation into many multiplex detection assays. Technology is beneficial to end users and therefore it is utilized in various pharmaceutical, clinical and research laboratories not only for nucleic acid assays but also for protein assay formats. On the market, there are available few commercial detection panels, however, these are mainly meant for major clinically important pathogens, infectious diseases or human health. Our research has been focused to fill in the market gaps and develop an in-house assay suitable for detection of genomic DNA of food-borne pathogens (e.g. Trichinella spiralis, Toxoplasma gondii, Giardia intestinalis, Yersinia enterocolitica, Campylobacter jejuni, Salmonella enterica) in various food-related matrices and design the diagnostic system based on the recombinant proteins characteristic for pathogen infections (e.g. Trichinella spp.). Up to this date, we have optimized the working protocol for highly specific multiplex oligonucleotide ligation PCR (MOL-PCR) together with MagPix analysis intended for multiplex detection panel for food-borne pathogens, which is currently still being expanded. The protein assay is under optimization.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů