Potential cytotoxic risk and chronical inflammation after exposure to graphene platelets

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000070" target="_blank" >RIV/00027162:_____/18:N0000070 - isvavai.cz</a>

Výsledek na webu

<a href="http://eventclass.org/contxt_eurotox2018/online-program/session?s=PV+2#e380" target="_blank" >http://eventclass.org/contxt_eurotox2018/online-program/session?s=PV+2#e380</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxlet.2018.06.899" target="_blank" >10.1016/j.toxlet.2018.06.899</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Potential cytotoxic risk and chronical inflammation after exposure to graphene platelets

Popis výsledku v původním jazyce

Purpose. Graphene represent two-dimensional (2D) nanomaterial with unique physical and chemical properties. Unlike graphene oxide less is known about biological effects of other derivatives such as graphene platelets and sheets (GPs) which workers are exposed during manufacturing. These non-biodegradable materials could pose a risk especially for respiratory system after exposure by inhalation. It has been proved that GPs of a certain size can be delivered beyond the ciliated airways and deposit in alveoli. There are internalized mostly by alveoli macrophages or simply persists in intercellular spaces, where can physically interact with other cells. This may lead to inflammation, disruption off cell metabolism and tissue damage. This study represents preliminary data of possible interactions between two different commercially available GPs and selected monocyte/macrophage cell lines. Methods. Dynamic light scattering method and Transmission Electron Microscopy (TEM) were used for characterization of GPs. THP-1 cells, THP-1 null cells, RAW BLUE cells and primary monocytes were used for experiments. The viability of cells was evaluated via WST-1 and LDH assays after 24, 48 and 72 hours of exposition. Localization of GPs was detected by TEM and confocal microscopy. Potential immune response was detected by activation of inflammasome NLRP3 test. Results. TEM confirmed presence of both GPs in cytoplasm after 24h, but no particles were located in nucleus. First results have shown that both GPs caused neither acute cytotoxicity nor activation of NLRP3 after 24h as well as 48h of exposition. However, levels of LDH which is released through damaged cell membrane increased with GPs concentration after 48h and 72h exposition of RAW BLUE cells. Interestingly, there was no increase of LDH after exposition of activated THP-1 cells and monocytes.

Název v anglickém jazyce

Potential cytotoxic risk and chronical inflammation after exposure to graphene platelets

Popis výsledku anglicky

Purpose. Graphene represent two-dimensional (2D) nanomaterial with unique physical and chemical properties. Unlike graphene oxide less is known about biological effects of other derivatives such as graphene platelets and sheets (GPs) which workers are exposed during manufacturing. These non-biodegradable materials could pose a risk especially for respiratory system after exposure by inhalation. It has been proved that GPs of a certain size can be delivered beyond the ciliated airways and deposit in alveoli. There are internalized mostly by alveoli macrophages or simply persists in intercellular spaces, where can physically interact with other cells. This may lead to inflammation, disruption off cell metabolism and tissue damage. This study represents preliminary data of possible interactions between two different commercially available GPs and selected monocyte/macrophage cell lines. Methods. Dynamic light scattering method and Transmission Electron Microscopy (TEM) were used for characterization of GPs. THP-1 cells, THP-1 null cells, RAW BLUE cells and primary monocytes were used for experiments. The viability of cells was evaluated via WST-1 and LDH assays after 24, 48 and 72 hours of exposition. Localization of GPs was detected by TEM and confocal microscopy. Potential immune response was detected by activation of inflammasome NLRP3 test. Results. TEM confirmed presence of both GPs in cytoplasm after 24h, but no particles were located in nucleus. First results have shown that both GPs caused neither acute cytotoxicity nor activation of NLRP3 after 24h as well as 48h of exposition. However, levels of LDH which is released through damaged cell membrane increased with GPs concentration after 48h and 72h exposition of RAW BLUE cells. Interestingly, there was no increase of LDH after exposition of activated THP-1 cells and monocytes.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20101 - Civil engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Toxicology Letters

ISBN

—

ISSN

0378-4274

e-ISSN

—

Počet stran výsledku

1

Strana od-do

S204-S204

Název nakladatele

ELSEVIER IRELAND LTD

Místo vydání

—

Místo konání akce

Brussels

Datum konání akce

2. 9. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000454045100561