Activation of NLRP3 inflammasome as a key indicator of carbon nanotubes proinflammatory potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000225" target="_blank" >RIV/00027162:_____/19:N0000225 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Activation of NLRP3 inflammasome as a key indicator of carbon nanotubes proinflammatory potential

Popis výsledku v původním jazyce

The aim of our study was to evaluate pro-inflammatory potential of multi-walled carbon nanotubes (MWCNTs). Despite the fact, that some of these nanotubes are not acutely cytotoxic in vitro, mechanical disruption of some organelles like lysosomes can trigger NLRP3 assembly leading to production of proinflammatory cytokines IL-1β and IL-18. We regard NLRP3 activation as an essential step in induction of chronic inflammation in vivo. Pristine PMWCNTs and functionalized NDMWCNTs and COOHMWCNTs were tested in our study. Detailed observation of shape and length of MWCNTs was assessed using electron microscopy. Human monocyte-derived cell lines THP-1, THP1-null and THP1-defNLRP3 were used as in vitro model. Cell viability was evaluated via LDH assay. Localization of MWCNTs was detected by electron microscopy. Activation of NLRP3 and release of IL-1β was measured by cell-based assay using HEKBlue ™ IL-1β cells. ELISA kits were used for detection of other cytokines in supernatants. After 24h, MWCNTs were located as free particles in the whole cytoplasm. Rarely, some individual tubes were observed to reach the nucleus membrane. Interestingly, while all types of MWCNTs activated NLRP3, only COOHMWCNTs caused release of proinflammatory cytokines IL-6 and TNF-α. Increased LDH levels showed higher cytotoxicity in the case of functionalized MWCNTs only.

Název v anglickém jazyce

Activation of NLRP3 inflammasome as a key indicator of carbon nanotubes proinflammatory potential

Popis výsledku anglicky

The aim of our study was to evaluate pro-inflammatory potential of multi-walled carbon nanotubes (MWCNTs). Despite the fact, that some of these nanotubes are not acutely cytotoxic in vitro, mechanical disruption of some organelles like lysosomes can trigger NLRP3 assembly leading to production of proinflammatory cytokines IL-1β and IL-18. We regard NLRP3 activation as an essential step in induction of chronic inflammation in vivo. Pristine PMWCNTs and functionalized NDMWCNTs and COOHMWCNTs were tested in our study. Detailed observation of shape and length of MWCNTs was assessed using electron microscopy. Human monocyte-derived cell lines THP-1, THP1-null and THP1-defNLRP3 were used as in vitro model. Cell viability was evaluated via LDH assay. Localization of MWCNTs was detected by electron microscopy. Activation of NLRP3 and release of IL-1β was measured by cell-based assay using HEKBlue ™ IL-1β cells. ELISA kits were used for detection of other cytokines in supernatants. After 24h, MWCNTs were located as free particles in the whole cytoplasm. Rarely, some individual tubes were observed to reach the nucleus membrane. Interestingly, while all types of MWCNTs activated NLRP3, only COOHMWCNTs caused release of proinflammatory cytokines IL-6 and TNF-α. Increased LDH levels showed higher cytotoxicity in the case of functionalized MWCNTs only.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Farmakologie, Imunoterapie, nanoToxikologie)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

EUROPEAN JOURNAL OF IMMUNOLOGY

ISBN

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ISSN

0014-2980

e-ISSN

1521-4141

Počet stran výsledku

1

Strana od-do

601-601

Název nakladatele

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Místo vydání

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Místo konání akce

Beijing, PEOPLES R CHINA

Datum konání akce

19. 10. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000490026901573