Determining spatial distribution of interleukin-1β as an infection marker in pulmonary porcine tissues

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917111" target="_blank" >RIV/62156489:43210/19:43917111 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determining spatial distribution of interleukin-1β as an infection marker in pulmonary porcine tissues

Popis výsledku v původním jazyce

Interleukin-1β (IL-1β) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1β could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1β for IL-1β detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1β. This IL-1β tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1β in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

Název v anglickém jazyce

Determining spatial distribution of interleukin-1β as an infection marker in pulmonary porcine tissues

Popis výsledku anglicky

Interleukin-1β (IL-1β) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1β could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1β for IL-1β detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1β. This IL-1β tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1β in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019: Proceedings of International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

459-464

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000576735500082