Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU140135" target="_blank" >RIV/00216305:26620/19:PU140135 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

Popis výsledku v původním jazyce

Interleukin-1 beta (IL-1 beta) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1 beta could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1 beta for IL-1 beta detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1 beta. This IL-1 beta tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1 beta in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

Název v anglickém jazyce

Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

Popis výsledku anglicky

Interleukin-1 beta (IL-1 beta) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1 beta could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1 beta for IL-1 beta detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1 beta. This IL-1 beta tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1 beta in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019: Proceedings of International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

459-464

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

000576735500082